The table below provides information about integral membrane proteins whose structures have been determined by solution-state (blue background), oriented solid-state NMR (grey background) or magic-angle spinning solid-state NMR (pink background), to a resolution sufficient to have resulted in a file deposited at the RCSB Protein Data Bank.
When possible, the table below also provides chemical shift assignments deposited at the Biological Magnetic Resonance Data Bank, protein size and oligomeric state, production source, reconstitution medium and primary reference.
Last database update : 23 february 2010
Unique proteins in database : 34
Number of coordinate files in database : 46
For more membrane protein structures : Steve White's Website
For more protein structures: determined by solid state NMR
| Protein | PDB Entry |
BMRB Entry |
Oligomer Size/State |
Production Source |
Reconstitution Medium |
PubMed Reference |
|---|---|---|---|---|---|---|
| TRANSMEMBRANE PROTEINS: BETA-BARREL | ||||||
|
OmpA
|
Escherichia coli | |||||
|
Escherichia coli | |||||
|
Escherichia coli | |||||
|
PagP
|
Escherichia coli | |||||
|
OmpX Without H-bond constraints: 1Q9G |
Escherichia coli | |||||
|
OmpG |
Escherichia coli | |||||
|
human VDAC-1
|
|
Escherichia coli | ||||
| TRANSMEMBRANE PROTEINS: ALPHA-HELICAL | ||||||
|
Gramicidin |
synthetic | Ketchem et al. (1993) | ||||
|
human Glycophorin A transmembrane domain |
Escherichia coli | |||||
AchR M2 channel |
Escherichia coli | Opella et al. (1999) | ||||
|
a-factor receptor M6 |
synthetic | Valentine et al. (2001) | ||||
|
Influenza M2 channel residues 22-46 |
synthetic | Wang et al. (2001) | ||||
|
8 kDa/4 | synthetic | lipids | Hu et al. (2007) | ||
|
Escherichia coli | |||||
|
synthetic | Cady et al. (2009) and Cady et al. (2010) |
||||
|
fd coat protein |
Enterobacteria phage fd | lipids | Marassi and Opella (2003) | |||
|
HIV Vpu channel |
Escherichia coli | Park et al. (2003) | ||||
|
Mistic membrane-integrating protein Note: This is not a constitutive membrane protein. It is included here because of general interest |
Escherichia coli | |||||
|
Mer F |
Escherichia coli | |||||
|
KcsA |
6728 (methyls) |
Escherichia coli | ||||
| 30 kDa/5 | Escherichia coli | DPC | ||||
|
2KB7 | 30 kDa/5 | Escherichia coli | DPC and lipids | Traaseth et al. (2009) | |
|
Zetazeta transmembrane domain |
Escherichia coli | |||||
| 10 kDa/2 | Escherichia coli | |||||
|
human FXYD1 (Na,K-ATPase Regulatory Protein) |
Escherichia coli | |||||
|
human FXYD4 (CHIF) |
7 kDa/1 | Escherichia coli | ||||
ErbB2 transmembrane segment |
Escherichia coli | |||||
|
EphA1 transmembrane domain |
8 kDa/2 | Escherichia coli | bicelles | |||
|
human KCNE1
|
16 kDa/1 | Escherichia coli | ||||
|
DsbB and DsbB-ubiquinone complex
|
18 kDa/1 | Escherichia coli | ||||
|
Integrin alphaIIb-beta3 |
9 kDa/2 | Escherichia coli | ||||
| i3 intracellular loop of the vasopressin V2 receptor | 6 kDa/1 | synthetic | DPC | Bellot et al. (2009) | ||
| G-protein-coupled receptor double transmembrane fragment | 9 kDa/1 | Escherichia coli | LPPG | Neumoin et al. (2009) | ||
Cytosolic domain of C. elegans mechanosensory ion channel subunit MEC-4 |
2K2B | 13 kDa/1 | Escherichia coli | Zwittergent | Everett et al. (2009) | |
|
Diacylglycerol Kinase (DAGK) |
Escherichia coli | |||||
|
Rv1761c from Mycobacterium tuberculosis |
Escherichia coli | |||||
| human alpha-defensin HNP-1 |
3 kDa/1 |
Escherichia coli | microcrystalised outside the lipid membrane |
Zhang et al. (2009) | ||
Arora A, Abildgaard F, Bushweller JH, & Tamm LK (2001). Structure of outer membrane protein A transmembrane domain by NMR spectroscopy. Nature Structural Biol. 8:334-338.
Bayrhuber, M., Meins, T., Habeck, M., Becker, S., Giller, K., Villinger, S., Vonrhein, C., Griesinger, C., Zweckstetter, M., Zeth, K. (2008) Structure of the Human Voltage-Dependent Anion Channel. Proc.Natl.Acad.Sci.USA 105:15370-15375
Bellot, G., Granier, S., Bourguet, W., Seyer, R., Rahmeh, R., Mouillac, B., Pascal, R., Mendre, C., Demene, H. (2009) Structure of the third intracellular loop of the vasopressin V2 receptor and conformational changes upon binding to gC1qR J. Mol. Biol. 388:491–507
Bocharov, E.V., Pustovalova, Y.E., Pavlov, K.V., Volynsky, P.E., Goncharuk, M.V., Ermolyuk, Y.S., Karpunin, D.V., Schulga, A.A., Kirpichnikov, M.P., Efremov, R.G., Maslennikov, I.V., Arseniev, A.S. (2007) Unique Dimeric Structure of Bnip3 Transmembrane Domain Suggests Membrane Permeabilization as a Cell Death Trigger. J Biol Chem. 282:16256-16266
Bocharov, E.V., Mineev, K.S., Volynsky, P.E., Ermolyuk, Y.S., Tkach, E.N., Sobol, A.G., Chupin, V.V., Kirpichnikov, M.P., Efremov, R.G., Arseniev, A.S. (2008) Spatial Structure of the Dimeric Transmembrane Domain of the Growth Factor Receptor ErbB2 Presumably Corresponding to the Receptor Active State J.Biol.Chem. 283:6950-6956
Bocharov, E.V., Mayzel, M.L., Volynsky, P.E., Goncharuk, M.V., Ermolyuk, Y.S., Schulga, A.A., Artemenko, E.O., Efremov, R.G., Arseniev, A.S. (2008) Spatial structure and pH-dependent conformational diversity of dimeric transmembrane domain of the receptor tyrosine kinase EphA1. J.Biol.Chem. 283:29385-29395
Cady, S.D., Mishanina, T.V., Hong, M. (2009) Structure of amantadine-bound M2 transmembrane peptide of influenza A in lipid bilayers from magic-angle-spinning solid-state NMR: the role of Ser31 in amantadine binding J. Mol. Biol. 385:1127-1141
Cady, S.D., Schmidt-Rohr K., Wang J., Soto C.S., Degrado W.F., Hong, M. (2010) Structure of the amantadine binding site of influenza M2 proton channels in lipid bilayers Nature 463:689-692
Call, M.E., Schnell, J.R., Xu, C., Lutz, R.A., Chou, J.J., Wucherpfennig, K.W. (2006) The structure of the zetazeta transmembrane dimer reveals features essential for its assembly with the T cell receptor. Cell 127:355-368
Fernández C, Hilty C, Wider G, Guntert P, & Wuthrich K (2004). NMR structure of the integral membrane protein OmpX. J Mol Biol. 336:1211-1221.
Franzin, C.M., Teriete, P., Marassi, F.M. (2007) Structural similarity of a membrane protein in micelles and membranes J.Am.Chem.Soc. 129:8078-8079
Hiller, S., Garces, R.G., Malia, T.J., Orekhov, V.Y., Colombini, M., Wagner, G. (2008) Solution structure of the integral human membrane protein VDAC-1 in detergent micelles. Science 321:1206-1210
Howell, S.C., Mesleh, M.F., Opella, S.J. (2005) NMR Structure Determination of a Membrane Protein with Two Transmembrane Helices in Micelles: Merf of the Bacterial Mercury Detoxification System Biochemistry 44:5196-5206
Hu, J., Asbury, T., Achuthan, S., Li, C., Bertram, R., Quine, J.R., Fu, R., Cross, T.A. (2007) Backbone structure of the amantadine-blocked trans-membrane domain m2 proton channel from influenza a virus Biophys. J. 92:4335-4343
Hwang PM, Choy WY, Lo EI, Chen L, Forman-Kay JD, Raetz CR, Privé GG, Bishop RE & Kay LE (2002). Solution structure and dynamics of the outer membrane enzyme PagP by NMR. Proc Natl Acad Sci USA 99:13560-13565.
Johansson MU, Alioth S, Hu K, Walser R, Koebnik R, & Pervushin K (2007). A minimal transmembrane β-barrel platform protein studied by nuclear magnetic resonance. Biochemistry 46:1128-1140.
Ketchem, R.R., W. Hu, and T.A. Cross (1993) High-resolution conformation of gramicidin A in a lipid bilayer by solid-state NMR. Science 261:1457-1460
Lau, T.L., Kim, C., Ginsberg, M.H., Ulmer, T.S. (2009) The structure of the integrin alphaIIbbeta3 transmembrane complex explains integrin transmembrane signalling. EMBO J. 28:1351-1361
Liang B & Tamm LK (2007). Structure of outer membrane protein G by solution NMR Spectroscopy Proc Natl Acad Sci USA 104:16140-16145
MacKenzie, K.R., Prestegard, J.H., Engelman, D.M. (1997) A transmembrane helix dimer: structure and implications. Science 276:131-133
Marassi, FM, & Opella, SJ (2003) Simultaneous assignment and structure determination of a membrane protein from NMR orientational restraints. Protein Sci. 12:403-411
Neumoin, A., Cohen, L.S., Arshava, B., Tantry, S., Becker, J.M., Zerbe, O., Naider, F. (2009) Structure of a double transmembrane fragment of a G-protein-coupled receptor in micelles Biophys. J. 96:3187-3196
Opella, S.J., F.M. Marassi, J.J. Gesell, A.P. Valente, Y. Kim, M. Oblatt-Montal, and M. Montal (1999) Structures of the M2 channel-lining segments from nicotinic acetylcholine and NMDA receptors by NMR spectroscopy. Nat. Struct. Biol. 6:374-379
Oxenoid K & Chou JJ (2005). The structure of phospholamban pentamer reveals a channel-like architecture in membranes Proc Natl Acad Sci USA 102:10870-10875
Page, R.C., Lee, S., Moore, J.D., Opella, S.J., Cross, T.A. (2009) Backbone structure of a small helical integral membrane protein: A unique structural characterization Protein Sci 18:134-146
Park, S.H., A.A. Mrse, A.A. Nevzorov, M.F. Mesleh, M. Oblatt-Montal, M. Montal, and S.J. Opella (2003) Three-dimensional structure of the channel-forming trans-membrane domain of virus protein "u" (Vpu) from HIV-1. J. Mol. Biol. 333:409-424
Renault, M., Saurel, O., Czaplicki, J., Demange, P., Gervais, V., Lohr, F., Reat, V., Piotto, M., Milon, A. (2009) Solution state NMR structure and dynamics of KpOmpA, a 210 residue transmembrane domain possessing a high potential for immunological applications. J. Mol. Biol. 385:117-130
Roosild TP, Greenwald J, Vega M, Castronovo S, Riek R, & Choe S (2005). NMR structure of Mistic, a membrane-integrating protein for membrane protein expression. Science 307:1317-1321
Schnell JR & Chou JJ (2008). Structure and mechanism of the M2 proton channel of influenza A virus. Nature 451:591-595
Teriete P, Franzin CM, Choi J, & Marassi FM (2007). Structure of the Na,K-ATPase regulatory protein FXYD1 in micelles. Biochemistry 46:6774-6783
Traaseth, N.J., Shi, L., Verardi, R., Mullen, D.G., Barany, G., Veglia, G. (2009) Structure and topology of monomeric phospholamban in lipid membranes determined by a hybrid solution and solid-state NMR approach. Proc. Natl. Acad. Sci.USA 106:10165-10170
Valentine, K.G., S.F. Liu, F.M. Marassi, G. Veglia, S.J. Opella, F.X. Ding, S.H. Wang, B. Arshava, J.M. Becker, and F. Naider (2001) Structure and topology of a peptide segment of the 6th transmembrane domain of the Saccharomyces cerevisae alpha-factor receptor in phospholipid bilayers. Biopolymers 59:243-256
Van Horn, W.D., Kim, H.J., Ellis, C.D., Hadziselimovic, A., Sulistijo, E.S., Karra, M.D., Tian, C., Sonnichsen, F.D., Sanders, C.R. (2009) Solution nuclear magnetic resonance structure of membrane-integral diacylglycerol kinase. Science 324:1726-1729
Wang, J., S. Kim, F. Kovacs, and T.A. Cross (2001) Structure of the transmembrane region of the M2 protein H(+) channel. Protein Sci 10:2241-2250
Yu, L., Sun, C., Song, D., Shen, J., Xu, N., Gunasekera, A., Hajduk,
P.J., Olejniczak, E.T. (2005) Nuclear magnetic resonance structural
studies of a potassium channel-charybdotoxin complex. Biochemistry 44:15834-15841
Zhang Y., Doherty T., Li J., Lu W., Barinka C., Lubkowski J., Hong M. (2010) Resonance Assignment and Three-Dimensional Structure Determination of a Human a-Defensin, HNP-1, by solid-state NMR J. Mol. Biol.
Zhou, Y., Cierpicki, T., Flores Jimenez, R.H., Lukasik, S.M., Ellena, J.F., Cafiso, D.S., Kadokura, H., Beckwith, J., Bushweller, J.H. (2008) NMR Solution Structure of the Integral Membrane Enzyme DsbB: Functional Insights into DsbB-Catalyzed Disulfide Bond Formation. Mol.Cell 31:896-908