MEMBRANE PROTEINS OF KNOWN STRUCTURE BY NMR

The table below provides information about integral membrane proteins whose structures have been determined by solution-state NMR, oriented solid-state NMR or magic-angle spinning solid-state NMR, to a resolution sufficient to have resulted in a file deposited at the RCSB Protein Data Bank.

When possible, the table below also provides chemical shift assignments deposited at the Biological Magnetic Resonance Data Bank, protein size and oligomeric state, production source, reconstitution medium and primary reference.

Please help me to keep this table up to date by sending me missing information at: dror.warschawski_at_ibpc.fr

Last database update : 16 November 2011

Unique proteins in database : 66

Number of coordinate files in database : 98

For more membrane protein structures : Steve White's Website

For more protein structures: determined by solid state NMR


Protein	PDB Entry	BMRB Entry	Oligomer Size/State	Production Source	Reconstitution Medium	NMR Method	PubMed Reference
TRANSMEMBRANE PROTEINS: BETA-BARREL
OmpA	1G90 2GE4		19 kDa/1	Escherichia coli	DPC (600 mM; 50°C)	solution	Arora et al. (2001) Cierpicki et al. (2006)
OmpA with four shortened loops Also called beta-barrel platform (BBP)	2JMM	15045	16 kDa/1	Escherichia coli	DHPC (37°C)	solution	Johansson et al. (2007)
Kp OmpA	2K0L	15651	22 kDa/1	Escherichia coli	DHPC (300 mM; 40°C)	solution	Renault et al. (2009)
PagP	1MM4 1MM5	5557 6234	20 kDa/1	Escherichia coli	DPC (500 mM; 45°C) and OG (200 mM; 45°C)	solution	Hwang et al. (2002)
OmpX Without H-bond constraints:	1ORM 1Q9F 1Q9G	4936 15201	16 kDa/1	Escherichia coli	DHPC (200 mM; 30°C)	solution	Fernández et al. (2001) Fernández et al. (2004)
OmpG	2JQY	15426	28 kDa/1	Escherichia coli	DPC (40°C)	solution	Liang & Tamm (2007)
human VDAC-1	2K4T 2JK4		32 kDa/1	Escherichia coli	LDAO (400 mM; 30°C and 300 mM; 37°C)	solution	Hiller et al. (2008) Bayrhuber et al. (2008)

TRANSMEMBRANE PROTEINS: ALPHA-HELICAL
Gramicidin	1MAG		4 kDa/2	synthetic	lipids	oriented	Ketchem et al. (1993)
human Glycophorin A transmembrane domain	1AFO 2KPE 2KPF		4 kDa/2	Escherichia coli	DPC (40°C) DPC bicelles	solution	MacKenzie et al. (1997)
fd coat protein	1FDM	5815	5 kDa/1	Escherichia coli	SDS	solution	Almeida and Opella (1997)
fd coat protein	1MZT	5815	5 kDa/1	Escherichia coli	lipids	oriented	Marassi and Opella (2003)
Subunit c of the F1Fo ATP synthase from E Coli assignment was also performed in MAS NMR on a precipitate and in solution NMR in LPPG micelles	1A91 1C0V	4146 10021 15564	8 kDa/1	Escherichia coli	Chloroform, Methanol, Water	solution	Girvin et al. (1998) Kobayashi et al. (2006) Zhang et al. (2008)
Subunit b of the F1Fo ATP synthase from E Coli	1B9U		4 kDa/1	synthetic	Chloroform, Methanol, Water	solution	Dmitriev et al. (1999)
A1C12 subcomplex of the F1Fo ATP synthase from E Coli at pH 5 and pH 8	1C17 1C99	4151 6021	119 kDa/12	Escherichia coli	Chloroform, Methanol, Water	solution	Rastogi and Girvin (1999) Dmitriev et al. (2004)
D24N61 mutant of subunit c of the F1Fo ATP synthase from E Coli	1L6T	5326	8 kDa/1	Escherichia coli	Chloroform, Methanol, Water	solution	Dmitriev et al. (2002)
Subunit c of the F1Fo ATP synthase from bacillus PS3	1WUO	6489	7 kDa/1	Escherichia coli	Chloroform, Methanol, Water	solution	Nakano et al. (2006)
Neuronal acetylcholine receptor M2 channel	1CEK		3 kDa/1	Escherichia coli	lipids	oriented	Opella et al. (1999)
Second transmembrane domain of the neuronal acetylcholine receptor beta 2 subunit	2K59		3 kDa/1	synthetic	DPC	solution	Yushmanov et al. (2003)
First transmembrane domain of the neuronal acetylcholine receptor beta 2 subunit	2K58		4 kDa/1	synthetic	DPC	solution	Bondarenko et al. (2007)
Transmembrane domain of the neuronal acetylcholine receptor beta 2 subunit	2KSR	101528	18 kDa/1	Escherichia coli	Hexafluoro- isopropanol, Water (40°C)	solution	Bondarenko et al. (2010)
alpha-factor receptor M6	1PJD		2 kDa/1	synthetic	lipids	oriented	Valentine et al. (2001)
Influenza M2 proton channel residues 22-46	1NYJ		11 kDa/4	synthetic	lipids (DMPC, 30°C)	oriented	Wang et al. (2001)
Influenza M2 proton channel residues 22-46 with amantadine	2H95		8 kDa/4	synthetic	lipids (DMPC, DMPG, 25°C)	oriented	Hu et al. (2007)
Influenza M2 proton channel residues 18-60 with rimantadine inhibitor	2RLF		20 kDa/4	Escherichia coli	DHPC (300 mM; 30°C)	solution	Schnell & Chou. (2008)
S31N mutant of influenza M2 proton channel residues 18-60 with rimantadine inhibitor	2KIH		20 kDa/4	Escherichia coli	DHPC (150 mM; 30°C)	solution	Pielak et al. (2009)
BV27A mutant of influenza M2 proton channel residues 18-60 with rimantadine inhibitor	2KWX		18 kDa/4	Escherichia coli	DHPC (150 mM; 30°C)	solution	Pielak & Chou (2010)
Influenza M2 proton channel residues 22-46 with amantadine	2KAD 2KQT	16020 16612	11 kDa/4	synthetic	lipids (DLPC) lipids (DMPC) -30°C	MAS and oriented	Cady et al. (2009) Cady et al. (2010)
Influenza M2 proton channel residues 22-62	2L0J		20 kDa/4	Escherichia coli	lipids (DOPC, DOPC, 30°C)	oriented	Sharma et al. (2010)
Rhodobacter sphaeroides Light Harvesting 1 beta Subunit	1JO5	4949	5 kDa/1	Rhodobacter sphaeroides	Zwittergent 3-12	solution	Sorgen et al. (2002)
HIV Vpu channel	1PJE 1PI7 1PI8		4 kDa/1	Escherichia coli	lipids	oriented	Park et al. (2003)
Mer F	1WAZ		6 kDa/1	Escherichia coli	SDS (500 mM; 60°C)	solution	Howell et al. (2005)
Mer F	2H3O		6 kDa/1	Escherichia coli	bicelles, 40°C	oriented	De Angelis et al. (2006)
KcsA with Charybdotoxin	2A9H	6728 (methyls)	71 kDa/4	Escherichia coli	DPC (80 mM, 42°C)	solution	Yu et al. (2005)
Stannin	1ZZA	6715	14 kDa/1	Escherichia coli	SDS	solution and oriented	Buck-Koehntop et al. (2005)
human Phospholamban	1ZLL 1FJK 1FJP	4907	30 kDa/5	Escherichia coli	DPC (200 mM; 30°C)	solution	Oxenoid and Chou (2005)
human Phospholamban	2KB7 2KYV		30 kDa/5	Escherichia coli	DPC (300 mM; 37°C) and lipids	solution, oriented and MAS	Traaseth et al. (2009) Verardi et al. (2011)
ZetaZeta transmembrane domain	2HAC		4 kDa/2	Escherichia coli	DPC/SDS:5/1 (150 mM DPC; 30 mM SDS; 30°C)	solution	Call et al. (2006)
Protegrin-1	1ZY6		4 kDa/2	synthetic	lipids (POPC)	MAS	Mani et al. (2006)
Bnip3 transmembrane domain	2J5D 2KA1 2KA2	7288 16012	10 kDa/2	Escherichia coli	bicelles (160 mM DHPC; 40 mM DMPC; 40°C)	solution	Bocharov et al. (2007)
human FXYD1 (Na,K-ATPase Regulatory Protein)	2JO1		8 kDa/1	Escherichia coli	SDS (500 mM; 40°C)	solution	Teriete et al. (2007)
human FXYD4 (CHIF)	2JP3		7 kDa/1	Escherichia coli	SDS	solution	Franzin et al. (2007)
ErbB2 transmembrane segment	2JWA	15231	9 kDa/2	Escherichia coli	bicelles (140 mM DHPC; 35 mM DMPC; 40°C)	solution	Bocharov et al. (2008)
Transmembrane domain heterodimer of ErbB1 and ErbB2 receptor tyrosine kinases	2KS1		9 kDa/1	Escherichia coli	bicelles (140 mM DHPC; 35 mM DMPC; 40°C)	solution	Mineev et al. (2010)
Dimeric ErbB3 transmembrane domain	2L9U	17488	9 kDa/2	Escherichia coli	DPC	solution	Mineev et al. (2011)
EphA1 transmembrane domain at pH 4.3 and 6.3	2K1K 2K1L	15728	8 kDa/2	Escherichia coli	bicelles (140 mM DHPC; 35 mM DMPC; 40°C)	solution	Bocharov et al. (2008)
EphA2 dimeric structure at pH 5	2K9Y	16005	9 kDa/2	Escherichia coli	bicelles (140 mM DHPC; 35 mM DMPC; 40°C)	solution	Bocharov et al. (2010)
human KCNE1	2K21		16 kDa/1	Escherichia coli	LMPG (80 mM; 40°C)	solution	Kang et al. (2008)
DsbB and DsbB-ubiquinone complex	2K73 2K74		18 kDa/1	Escherichia coli	DPC, 40°C	solution	Zhou et al. (2008)
Integrin alphaIIb-beta3	2KNC		15 kDa/1	Escherichia coli	Acetonitrile, Water, 25°C	solution	Yang et al. (2009)
Integrin alphaIIb-beta3 shorter construct	2K9J		9 kDa/1	Escherichia coli	bicelles (385 mM DHPC; 83 mM POPC; 41mM POPS; 30°C)	solution	Lau et al. (2009)
Integrin beta3 subunit	2KV9	16771	9 kDa/1	Escherichia coli	DPC	solution	Metcalf et al. (2010)
N-terminal domain of subunit E (E1-52) of A1AO ATP synthase from Methanocaldococcus jannaschii	2KK7	16354	6 kDa/1	Escherichia coli	Trifluoroethanol, Water	solution	Gayen et al. (2009)
i3 intracellular loop of the vasopressin V2 receptor	2JX4		6 kDa/1	synthetic	DPC	solution	Bellot et al. (2009)
Ste2p of yeast alpha-factor receptor, double transmembrane fragment	2K9P		9 kDa/1	Escherichia coli	LPPG (200 mM; 47°C)	solution	Neumoin et al. (2009)
Diacylglycerol Kinase (DAGK)	2KDC	15019 7340	43 kDa/3	Escherichia coli	DPC, 45°C	solution	Van Horn et al. (2009)
Rv1761c from Mycobacterium tuberculosis	2K3M	15774	17 kDa/1	Escherichia coli	DPC (150 mM; 50°C)	solution	Page et al. (2009)
Pf1 coat protein	2KLV	5877	5 kDa/1	Pseudomonas aeroginusa	DHPC (100 mM; 50°C)	solution	Park et al. (2009)
Pf1 coat protein	2KSJ	5877	5 kDa/1	Pseudomonas aeroginusa	DHPC, bicelles and lipids	solution and oriented	Park et al. (2010)
human alpha-defensin HNP-1	2KHT	16254	3 kDa/1	Escherichia coli	microcrystalised outside the lipid membrane	MAS	Zhang et al. (2009)
Influenza B proton channel	2KIX		15 kDa/4	Escherichia coli	DHPC (300 mM, 30°C)	solution	Wang et al. (2009)
human CD4	2KLU	15479	8 kDa/1	Escherichia coli	DPC	solution	Wittlich et al. (2010)
Sensory rhodopsin II	2KSY	16678	26 kDa/1	Escherichia coli	c7-DHPC (100 mM; 50°C)	solution	Gautier et al. (2010)
Histidine-Rich Peptide LAH4 (pH 6.1 and 4.1)	2KJO 2KJN	16332	3 kDa/1	synthetic	DPC	solution	Georgescu et al. (2010)
Transmembrane Segment VI of the NHE1 Isoform of the Na+/H+ Exchanger	2L0E		3 kDa/1	synthetic	DPC	solution	Tzeng et al. (2010)
Influenza hemagglutinin fusion domain	2KXA	16907	3 kDa/1	Escherichia coli	DPC	solution	Lorieau et al. (2010)
Membrane domain of E. coli histidine kinase receptor ArcB	2KSD		13 kDa/1	Cell Free Expression	LMPG	solution	Maslennikov et al. (2010)
Membrane domain of E. coli histidine kinase receptor QseC	2KSE		21 kDa/1	Cell Free Expression	LMPG	solution	Maslennikov et al. (2010)
Membrane domain of E. coli histidine kinase receptor KdpD	2KSF		11 kDa/1	Cell Free Expression	LMPG	solution	Maslennikov et al. (2010)
DAP12 transmembrane homodimer	2L34		7 kDa/2	Escherichia coli	SDS (25 mM) and DPC (250 mM), 30°C	solution	Call et al. (2010)
DAP12-NKG2C transmembrane heterotrimer	2L35		10 kDa/1	Escherichia coli	SDS (25 mM) and DPC (250 mM), 30°C	solution	Call et al. (2010)
Voltage-sensing domain of KvAP	2KYH	16957	16 kDa/1	Escherichia coli	c7-DHPC (5mM, 45°C)	solution	Butterwick and MacKinnon (2010)
Mitochondrial uncoupling protein 2	2LCK	17614	33 kDa/1	Escherichia coli	DPC, Cardiolipin, DMPC	solution	Berardi et al. (2011)

MEMBRANE ASSOCIATED PROTEINS (NOT TRANSMEMBRANE)
Mistic membrane-integrating protein	1YGM		13 kDa/1	Escherichia coli	LDAO	solution	Roosild et al. (2005)
*Cytosolic domain of C. elegans* mechanosensory ion channel subunit MEC-4**	2K2B	15699	13 kDa/1	Escherichia coli	Zwittergent	solution	Everett et al.
Mastoparan (membrane bound rather than transmembrane)	2CZP	10001	2 kDa	Escherichia coli	DPPC:DPPG=4:1	MAS	Todokoro et al. (2006)
Rv0899 from Mycobacterium tuberculosis	2KSM		14 kDa/1	Escherichia coli	no detergents	solution	Teriete et al. (2010)
Lipoprotein-17 domain	2KRT	16648	14 kDa/1	Escherichia coli	no detergents	solution	Mani et al. (2010)
Substance P	2KS9 2KSA 2KSB	20115 20116 20117	1 kDa/1	synthetic	isotropic bicelles	solution	Gayen et al. (2011)

MEMBRANE PROTEINS OF KNOWN STRUCTURE

DETERMINED BY NMR