Last database update : 1 october 2008
Unique proteins in database : 24
Number of coordinate files in database : 30
For more membrane protein structures : Steve White's Website
For other protein structures: determined by solid state NMR
| Protein | PDB Code | BMRB Entry | Get File | Get Image |
Reference (links are to PubMed) |
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| TRANSMEMBRANE PROTEINS: BETA-BARREL | |||||
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OmpG: Escherichia coli, solution-state NMR (DPC micelles)
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OmpA: Escherichia coli, solution-state NMR (DPC micelles)
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OmpA with four shortened loops: Escherichia coli, solution-state NMR (DHPC micelles). Also called β-barrel platform (BBP)
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OmpX: Escherichia coli, solution-state NMR (DPC micelles)
Without H-bond constraints: 1Q9G |
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PagP: Escherichia coli, solution-state NMR
(DPC micelles)
Structure in OG micelles: 1MM5 |
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human VDAC-1: Escherichia coli, solution-state NMR
(LDAO micelles)
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| TRANSMEMBRANE PROTEINS: ALPHA-HELICAL | |||||
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Mistic membrane-integrating protein: Bacillus subtilis, solution-state NMR
Note: This is not a constitutive membrane protein. It is included here because of general interest. |
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Influenza M2 proton channel: Bacterial expression system, solution-state NMR
with rimantadine inhibitor |
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ErbB2 transmembrane segment dimer: Escherichia coli, solution-state NMR |
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Zetazeta transmembrane dimer: Escherichia coli, solution-state NMR |
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human Glycophorin A dimeric transmembrane domain: Escherichia coli, solution-state NMR |
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Bnip3 transmembrane domain: Escherichia coli, solution-state NMR (isotropic bicelles) |
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EphA1tm transmembrane domain dimer at pH 4.3 and 6.3: Escherichia coli, solution-state NMR (isotropic bicelles)
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human KCNE1: Escherichia coli, solution-state NMR (LMPG micelles)
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DsbB and DsbB-ubiquinone complex: solution-state NMR
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Zhou et al. (2008)
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human Na,K-ATPase Regulatory Protein FXYD1: Escherichia coli, solution-state NMR (SDS micelles) |
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human FXYD4 (CHIF): Escherichia coli, solution-state NMR (SDS micelles) |
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Gramicidin: oriented solid-state NMR |
Ketchem et al. (1993) | ||||
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AchR M2 channel: oriented solid-state NMR |
Opella et al. (1999) | ||||
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Influenza M2 channel: oriented solid-state NMR |
Wang et al. (2001) | ||||
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a-factor receptor M6: oriented solid-state NMR |
Valentine et al. (2001) | ||||
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fd coat protein: oriented solid-state NMR |
Marassi and Opella (2003) | ||||
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HIV Vpu channel: oriented solid-state NMR |
Park et al. (2003) | ||||
The table above provides information about integral membrane proteins whose structures have been determined by solution-state or solid-state NMR, to a resolution sufficient to have resulted in a file deposited at the RCSB Protein Data Bank.
Arora A, Abildgaard F, Bushweller JH, & Tamm LK (2001). Structure of outer membrane protein A transmembrane domain by NMR spectroscopy. Nature Structural Biol. 8:334-338.
Bocharov, E.V., Pustovalova, Y.E., Pavlov, K.V., Volynsky, P.E., Goncharuk, M.V., Ermolyuk, Y.S., Karpunin, D.V., Schulga, A.A., Kirpichnikov, M.P., Efremov, R.G., Maslennikov, I.V., Arseniev, A.S. (2007) Unique Dimeric Structure of Bnip3 Transmembrane Domain Suggests Membrane Permeabilization as a Cell Death Trigger. J Biol Chem. 282:16256-16266
Bocharov, E.V., Mineev, K.S., Volynsky, P.E., Ermolyuk, Y.S., Tkach, E.N., Sobol, A.G., Chupin, V.V., Kirpichnikov, M.P., Efremov, R.G., Arseniev, A.S. (2008) Spatial Structure of the Dimeric Transmembrane Domain of the Growth Factor Receptor ErbB2 Presumably Corresponding to the Receptor Active State J.Biol.Chem. 283:6950-6956
Bocharov, E.V., Mayzel, M.L., Volynsky, P.E., Goncharuk, M.V., Ermolyuk, Y.S., Schulga, A.A., Artemenko, E.O., Efremov, R.G., Arseniev, A.S. (2008) Spatial structure and pH-dependent conformational diversity of dimeric transmembrane domain of the receptor tyrosine kinase EphA1. J.Biol.Chem.
Call, M.E., Schnell, J.R., Xu, C., Lutz, R.A., Chou, J.J., Wucherpfennig, K.W. (2006) The structure of the zetazeta transmembrane dimer reveals features essential for its assembly with the T cell receptor. Cell(Cambridge,Mass.) 127: 355-368
Fernández C, Hilty C, Wider G, Guntert P, & Wuthrich K (2004). NMR structure of the integral membrane protein OmpX. J Mol Biol. 336:1211-1221.
Franzin, C.M., Teriete, P., Marassi, F.M. (2007) Structural similarity of a membrane protein in micelles and membranes J.Am.Chem.Soc. 129:8078-8079
Hiller, S., Garces, R.G., Malia, T.J., Orekhov, V.Y., Colombini, M., Wagner, G. (2008) Solution structure of the integral human membrane protein VDAC-1 in detergent micelles. Science 321:1206-1210
Hwang PM, Choy WY, Lo EI, Chen L, Forman-Kay JD, Raetz CR, Privé GG, Bishop RE & Kay LE (2002). Solution structure and dynamics of the outer membrane enzyme PagP by NMR. Proc Natl Acad Sci USA 99:13560-13565.
Johansson MU, Alioth S, Hu K, Walser R, Koebnik R, & Pervushin K (2007). A minimal transmembrane β-barrel platform protein studied by nuclear magnetic resonance. Biochemistry 46:1128-1140.
Ketchem, R.R., W. Hu, and T.A. Cross (1993) High-resolution conformation of gramicidin A in a lipid bilayer by solid-state NMR. Science 261:1457-1460
Liang B & Tamm LK (2007). Structure of outer membrane protein G by solution NMR Spectroscopy Proc Natl Acad Sci USA 104:16140-16145.
MacKenzie, K.R., Prestegard, J.H., Engelman, D.M. (1997) A transmembrane helix dimer: structure and implications. Science 276: 131-133
Marassi, FM, & Opella, SJ (2003) Simultaneous assignment and structure determination of a membrane protein from NMR orientational restraints. Protein Sci. 12:403-411
Opella, S.J., F.M. Marassi, J.J. Gesell, A.P. Valente, Y. Kim, M. Oblatt-Montal, and M. Montal (1999) Structures of the M2 channel-lining segments from nicotinic acetylcholine and NMDA receptors by NMR spectroscopy. Nat. Struct. Biol. 6:374-379
Oxenoid K & Chou JJ (2005). The structure of phospholamban pentamer reveals a channel-like architecture in membranes Proc Natl Acad Sci USA 102:10870-10875
Park, S.H., A.A. Mrse, A.A. Nevzorov, M.F. Mesleh, M. Oblatt-Montal, M. Montal, and S.J. Opella (2003) Three-dimensional structure of the channel-forming trans-membrane domain of virus protein "u" (Vpu) from HIV-1. J. Mol. Biol. 333:409-424
Roosild TP, Greenwald J, Vega M, Castronovo S, Riek R, & Choe S (2005). NMR structure of Mistic, a membrane-integrating protein for membrane protein expression. Science 307:1317-1321.
Schnell JR & Chou JJ (2008). Structure and mechanism of the M2 proton channel of influenza A virus. Nature 451:591-595.
Teriete P, Franzin CM, Choi J, & Marassi FM (2007). Structure of the Na,K-ATPase regulatory protein FXYD1 in micelles. Biochemistry 46:6774-6783.
Valentine, K.G., S.F. Liu, F.M. Marassi, G. Veglia, S.J. Opella, F.X. Ding, S.H. Wang, B. Arshava, J.M. Becker, and F. Naider (2001) Structure and topology of a peptide segment of the 6th transmembrane domain of the Saccharomyces cerevisae alpha-factor receptor in phospholipid bilayers. Biopolymers 59:243-256
Wang, J., S. Kim, F. Kovacs, and T.A. Cross (2001) Structure of the transmembrane region of the M2 protein H(+) channel. Protein Sci 10:2241-2250