Last database update : 1 october 2008

Unique proteins in database : 24

Number of coordinate files in database : 30

For more membrane protein structures : Steve White's Website

For other protein structures: determined by solid state NMR

 
Protein PDB Code BMRB Entry Get File Get Image Reference
(links are to PubMed)
TRANSMEMBRANE PROTEINS: BETA-BARREL
OmpG: Escherichia coli, solution-state NMR (DPC micelles)
2JQY
Liang & Tamm (2007)
OmpA: Escherichia coli, solution-state NMR (DPC micelles)
1G90
Arora et al. (2001)
OmpA with four shortened loops: Escherichia coli, solution-state NMR (DHPC micelles). Also called β-barrel platform (BBP)
2JMM
Johansson et al. (2007)
OmpX: Escherichia coli, solution-state NMR (DPC micelles)
Without H-bond constraints: 1Q9G
1Q9F
Fernández et al. (2004)
PagP: Escherichia coli, solution-state NMR (DPC micelles)
Structure in OG micelles:  1MM5
1MM4
Hwang et al. (2002)
human VDAC-1: Escherichia coli, solution-state NMR (LDAO micelles)
2K4T

 
Hiller et al. (2008)

 

TRANSMEMBRANE PROTEINS: ALPHA-HELICAL
Mistic membrane-integrating protein: Bacillus subtilis, solution-state NMR
Note: This is not a constitutive membrane protein. It is included here because of general interest.
1YGM
Roosild et al. (2005)
Influenza M2 proton channel: Bacterial expression system, solution-state NMR
with rimantadine inhibitor
2RLF
Schnell & Chou. (2008)
Phospholamban homopentamer: Human sarcoplasmic reticulum, solution-state NMR
See also 1FJK and 1FJP.
1ZLL
ErbB2 transmembrane segment dimer: Escherichia coli, solution-state NMR
2JWA
Bocharov et al. (2008)
Zetazeta transmembrane dimer: Escherichia coli, solution-state NMR
2HAC
Call et al. (2006)
human Glycophorin A dimeric transmembrane domain: Escherichia coli, solution-state NMR
1AFO
MacKenzie et al. (1997)
Bnip3 transmembrane domain: Escherichia coli, solution-state NMR (isotropic bicelles)
2J5D
Bocharov et al. (2007)
EphA1tm transmembrane domain dimer at pH 4.3 and 6.3: Escherichia coli, solution-state NMR (isotropic bicelles)
2K1K and 2K1L
 and
and
Bocharov et al. (2008)
human KCNE1: Escherichia coli, solution-state NMR (LMPG micelles)
2K21
Kang et al. (2008)
DsbB and DsbB-ubiquinone complex: solution-state NMR
2K73 and 2K74
and
and
Zhou et al. (2008)
human Na,K-ATPase Regulatory Protein FXYD1: Escherichia coli, solution-state NMR (SDS micelles)
2JO1
Teriete et al. (2007)
human FXYD4 (CHIF): Escherichia coli, solution-state NMR (SDS micelles)
2JP3
Franzin et al. (2007)
Gramicidin: oriented solid-state NMR
1MAG
Ketchem et al. (1993)
AchR M2 channel: oriented solid-state NMR
1CEK
Opella et al. (1999)
Influenza M2 channel: oriented solid-state NMR
1NYJ
Wang et al. (2001)
a-factor receptor M6: oriented solid-state NMR
1PJD
Valentine et al. (2001)
fd coat protein: oriented solid-state NMR
1MZT
Marassi and Opella (2003)
HIV Vpu channel: oriented solid-state NMR
1PJE
Park et al. (2003)

Description of Table

The table above provides information about integral membrane proteins whose structures have been determined by solution-state or solid-state NMR, to a resolution sufficient to have resulted in a file deposited at the RCSB Protein Data Bank.

References

Arora A, Abildgaard F, Bushweller JH, & Tamm LK (2001). Structure of outer membrane protein A transmembrane domain by NMR spectroscopy. Nature Structural Biol. 8:334-338.

Bocharov, E.V., Pustovalova, Y.E., Pavlov, K.V., Volynsky, P.E., Goncharuk, M.V., Ermolyuk, Y.S., Karpunin, D.V., Schulga, A.A., Kirpichnikov, M.P., Efremov, R.G., Maslennikov, I.V., Arseniev, A.S. (2007) Unique Dimeric Structure of Bnip3 Transmembrane Domain Suggests Membrane Permeabilization as a Cell Death Trigger. J Biol Chem. 282:16256-16266

Bocharov, E.V., Mineev, K.S., Volynsky, P.E., Ermolyuk, Y.S., Tkach, E.N., Sobol, A.G., Chupin, V.V., Kirpichnikov, M.P., Efremov, R.G., Arseniev, A.S. (2008) Spatial Structure of the Dimeric Transmembrane Domain of the Growth Factor Receptor ErbB2 Presumably Corresponding to the Receptor Active State J.Biol.Chem. 283:6950-6956

Bocharov, E.V., Mayzel, M.L., Volynsky, P.E., Goncharuk, M.V., Ermolyuk, Y.S., Schulga, A.A., Artemenko, E.O., Efremov, R.G., Arseniev, A.S. (2008) Spatial structure and pH-dependent conformational diversity of dimeric transmembrane domain of the receptor tyrosine kinase EphA1. J.Biol.Chem.

Call, M.E., Schnell, J.R., Xu, C., Lutz, R.A., Chou, J.J., Wucherpfennig, K.W. (2006) The structure of the zetazeta transmembrane dimer reveals features essential for its assembly with the T cell receptor. Cell(Cambridge,Mass.) 127: 355-368

Fernández C, Hilty C, Wider G, Guntert P, & Wuthrich K (2004). NMR structure of the integral membrane protein OmpX. J Mol Biol. 336:1211-1221.

Franzin, C.M., Teriete, P., Marassi, F.M. (2007) Structural similarity of a membrane protein in micelles and membranes J.Am.Chem.Soc. 129:8078-8079

Hiller, S., Garces, R.G., Malia, T.J., Orekhov, V.Y., Colombini, M., Wagner, G. (2008) Solution structure of the integral human membrane protein VDAC-1 in detergent micelles. Science 321:1206-1210

Hwang PM, Choy WY, Lo EI, Chen L, Forman-Kay JD, Raetz CR, Privé GG, Bishop RE & Kay LE (2002). Solution structure and dynamics of the outer membrane enzyme PagP by NMR. Proc Natl Acad Sci USA 99:13560-13565.

Johansson MU, Alioth S, Hu K, Walser R, Koebnik R,  & Pervushin K (2007). A minimal transmembrane β-barrel platform protein studied by nuclear magnetic resonance. Biochemistry 46:1128-1140.

Ketchem, R.R., W. Hu, and T.A. Cross (1993) High-resolution conformation of gramicidin A in a lipid bilayer by solid-state NMR. Science 261:1457-1460

Liang B & Tamm LK (2007). Structure of outer membrane protein G by solution NMR Spectroscopy Proc Natl Acad Sci USA 104:16140-16145.

MacKenzie, K.R., Prestegard, J.H., Engelman, D.M. (1997) A transmembrane helix dimer: structure and implications. Science 276: 131-133

Marassi, FM, & Opella, SJ (2003) Simultaneous assignment and structure determination of a membrane protein from NMR orientational restraints. Protein Sci. 12:403-411

Opella, S.J., F.M. Marassi, J.J. Gesell, A.P. Valente, Y. Kim, M. Oblatt-Montal, and M. Montal (1999) Structures of the M2 channel-lining segments from nicotinic acetylcholine and NMDA receptors by NMR spectroscopy. Nat. Struct. Biol. 6:374-379

Oxenoid K & Chou JJ (2005). The structure of phospholamban pentamer reveals a channel-like architecture in membranes Proc Natl Acad Sci USA 102:10870-10875

Park, S.H., A.A. Mrse, A.A. Nevzorov, M.F. Mesleh, M. Oblatt-Montal, M. Montal, and S.J. Opella (2003) Three-dimensional structure of the channel-forming trans-membrane domain of virus protein "u" (Vpu) from HIV-1. J. Mol. Biol. 333:409-424

Roosild TP, Greenwald J, Vega M, Castronovo S, Riek R, & Choe S (2005). NMR structure of Mistic, a membrane-integrating protein for membrane protein expression. Science 307:1317-1321.

Schnell JR & Chou JJ (2008). Structure and mechanism of the M2 proton channel of influenza A virus. Nature 451:591-595.

Teriete P, Franzin CM, Choi J, & Marassi FM (2007). Structure of the Na,K-ATPase regulatory protein FXYD1 in micelles. Biochemistry 46:6774-6783.

Valentine, K.G., S.F. Liu, F.M. Marassi, G. Veglia, S.J. Opella, F.X. Ding, S.H. Wang, B. Arshava, J.M. Becker, and F. Naider (2001) Structure and topology of a peptide segment of the 6th transmembrane domain of the Saccharomyces cerevisae alpha-factor receptor in phospholipid bilayers. Biopolymers 59:243-256

Wang, J., S. Kim, F. Kovacs, and T.A. Cross (2001) Structure of the transmembrane region of the M2 protein H(+) channel. Protein Sci 10:2241-2250