MEMBRANE PROTEINS OF KNOWN STRUCTURE BY NMR

The table below provides information about integral membrane proteins whose structures have been determined by solution-state NMR, oriented solid-state NMR or magic-angle spinning solid-state NMR, to a resolution sufficient to have resulted in a file deposited at the RCSB Protein Data Bank.

When possible, the table below also provides chemical shift assignments deposited at the Biological Magnetic Resonance Data Bank, protein size and oligomeric state, production source, reconstitution medium and primary reference.

Please help me to keep this table up to date by sending me missing information at: dror.warschawski@ibpc.fr

Last database update : 1 june 2010

Unique proteins in database : 39

Number of coordinate files in database : 53

For more membrane protein structures : Steve White's Website

For more protein structures: determined by solid state NMR


Protein	PDB Entry	BMRB Entry	Oligomer Size/State	Production Source	Reconstitution Medium	NMR Method	PubMed Reference
TRANSMEMBRANE PROTEINS: BETA-BARREL
OmpA	1G90		19 kDa/1	Escherichia coli	DPC	solution	Arora et al. (2001)
OmpA with four shortened loops Also called beta-barrel platform (BBP)	2JMM	15045	16 kDa/1	Escherichia coli	DHPC	solution	Johansson et al. (2007)
Kp OmpA	2K0L	15651	22 kDa/1	Escherichia coli	DHPC	solution	Renault et al. (2009)
PagP	1MM4 1MM5	5557 6234	20 kDa/1	Escherichia coli	DPC and OG	solution	Hwang et al. (2002)
OmpX Without H-bond constraints: 1Q9G	1Q9F	4936 15201	16 kDa/1	Escherichia coli	DPC	solution	Fernández et al. (2004)
OmpG	2JQY	15426	28 kDa/1	Escherichia coli	DPC	solution	Liang & Tamm (2007)
human VDAC-1	2K4T 2JK4		32 kDa/1	Escherichia coli	LDAO	solution	Hiller et al. (2008) and Bayrhuber et al. (2008)

TRANSMEMBRANE PROTEINS: ALPHA-HELICAL
Gramicidin	1MAG		4 kDa/2	synthetic	lipids	oriented	Ketchem et al. (1993)
human Glycophorin A transmembrane domain	1AFO		4 kDa/2	Escherichia coli	DPC	solution	MacKenzie et al. (1997)
AchR M2 channel	1CEK		3 kDa/1	Escherichia coli	lipids	oriented	Opella et al. (1999)
a-factor receptor M6	1PJD		2 kDa/1	synthetic	lipids	oriented	Valentine et al. (2001)
Influenza M2 channel residues 22-46	1NYJ		11 kDa/4	synthetic	lipids	oriented	Wang et al. (2001)
Influenza M2 channel residues 22-46 with amantadine	2H95		8 kDa/4	synthetic	lipids	oriented	Hu et al. (2007)
Influenza M2 proton channel residues 18-60 with rimantadine inhibitor	2RLF		20 kDa/4	Escherichia coli	DHPC	solution	Schnell & Chou. (2008)
Influenza M2 channel residues 22-46 with amantadine	2KAD 2KQT	16020 16612	11 kDa/4	synthetic	lipids	MAS and oriented	Cady et al. (2009) and Cady et al. (2010)
fd coat protein	1MZT	5877	5 kDa/1	Enterobacteria phage fd	lipids	oriented	Marassi and Opella (2003)
HIV Vpu channel	1PJE 1PI7 1PI8		4 kDa/1	Escherichia coli	lipids	oriented	Park et al. (2003)
Mistic membrane-integrating protein Note: This is not a constitutive membrane protein. It is included here because of general interest	1YGM		13 kDa/1	Escherichia coli	LDAO	solution	Roosild et al. (2005)
Mer F	1WAZ		6 kDa/1	Escherichia coli	SDS	solution	Howell et al. (2005)
KcsA with Charybdotoxin	2A9H	6728 (methyls)	71 kDa/4	Escherichia coli	DPC	solution	Yu et al. (2005)
human Phospholamban See also 1FJK and 1FJP.	1ZLL	4907	30 kDa/5	Escherichia coli	DPC	solution	Oxenoid and Chou (2005)
human Phospholamban	2KB7		30 kDa/5	Escherichia coli	DPC and lipids	oriented	Traaseth et al. (2009)
Zetazeta transmembrane domain	2HAC		4 kDa/2	Escherichia coli	DPC/SDS:5/1	solution	Call et al. (2006)
MERFT	2H3O		6 kDa/1	Escherichia coli	bicelles	oriented	De Angelis et al. (2006)
Protegrin-1	1ZY6		4 kDa/2	synthetic	POPC	MAS	Mani et al. (2006)
Bnip3 transmembrane domain See also 2KA1 and 2KA2	2J5D	7288 16012	10 kDa/2	Escherichia coli	bicelles	solution	Bocharov et al. (2007)
human FXYD1 (Na,K-ATPase Regulatory Protein)	2JO1		8 kDa/1	Escherichia coli	SDS	solution	Teriete et al. (2007)
human FXYD4 (CHIF)	2JP3		7 kDa/1	Escherichia coli	SDS	solution	Franzin et al. (2007)
ErbB2 transmembrane segment	2JWA	15231	9 kDa/2	Escherichia coli	bicelles	solution	Bocharov et al. (2008)
EphA1 transmembrane domain at pH 4.3 and 6.3	2K1K 2K1L	15728	8 kDa/2	Escherichia coli	bicelles	solution	Bocharov et al. (2008)
human KCNE1	2K21		16 kDa/1	Escherichia coli	LMPG	solution	Kang et al. (2008)
DsbB and DsbB-ubiquinone complex	2K73 2K74		18 kDa/1	Escherichia coli	DPC	solution	Zhou et al. (2008)
Integrin alphaIIb-beta3	2K9J		9 kDa/2	Escherichia coli	bicelles	solution	Lau et al. (2009)
i3 intracellular loop of the vasopressin V2 receptor	2JX4		6 kDa/1	synthetic	DPC	solution	Bellot et al. (2009)
G-protein-coupled receptor double transmembrane fragment	2K9P		9 kDa/1	Escherichia coli	LPPG	solution	Neumoin et al. (2009)
*Cytosolic domain of C. elegans* mechanosensory ion channel subunit MEC-4** The structure is not transmembrane, just membrane associated.	2K2B	15699	13 kDa/1	Escherichia coli	Zwittergent	solution	Everett et al.
Diacylglycerol Kinase (DAGK)	2KDC	15019 7340	43 kDa/3	Escherichia coli	DPC	solution	Van Horn et al. (2009)
Rv1761c from Mycobacterium tuberculosis	2K3M	15774	17 kDa/1	Escherichia coli	DPC	solution	Page et al. (2009)
Pf1 major coat protein	2KLV		5 kDa/1	Pseudomonas aeroginusa	DHPC	solution	Park et al. (2009)
human alpha-defensin HNP-1	2KHT	16254	3 kDa/1	Escherichia coli	microcrystalised outside the lipid membrane	MAS	Zhang et al. (2009)
human CD4	2KLU	15479	8 kDa/1	Escherichia coli	DPC	solution	Wittlich et al. (2010)
Sensory rhodopsin II	2KSY	16678	26 kDa/1	Escherichia coli	DHPC	solution	Gautier et al. (2010)

MEMBRANE PROTEINS OF KNOWN STRUCTURE

DETERMINED BY NMR