The table below provides information about proteins whose structures have been determined by solid-state NMR, to a resolution sufficient to have resulted in a file deposited at the RCSB Protein Data Bank.
Last database update : 23 february 2010
Unique proteins in database : 18
Number of coordinate files in database : 23
For more membrane protein structures: determined by NMR
| Protein | PDB Entry |
BMRB Entry |
Oligomer Size/State |
Production Source |
NMR Method |
PubMed Reference |
|---|---|---|---|---|---|---|
| MICRO-CRYSTALLINE
PROTEINS |
||||||
| Spectrin
SH3 domain |
1M8M |
7 kDa/1 | Escherichia coli | MAS |
Castellani
et al. (2002) |
|
| Kaliotoxin |
4 kDa/1 | Escherichia coli | MAS |
|||
| beta1 immunoglobulin binding domain of protein G (GB1) |
6 kDa/1 | Escherichia coli | MAS |
|||
| Crh domain, swapped dimer | 2RLZ |
19 kDa/2 | Escherichia coli | MAS |
Loquet et al. (2008) | |
| Ubiquitin |
2JZZ |
9 kDa/1 | Escherichia coli | MAS |
Manolikas et al. (2008) |
|
| MMP-12 (Macrophage metalloelastase) |
2K9C 2KRJ |
17 kDa/1 | Escherichia coli | MAS |
Balayssac et al. (2008) and Bertini et al. (2010) |
|
| human alpha-defensin HNP-1 | 3 kDa/1 | Escherichia coli | MAS | Zhang et al. (2009) | ||
| PROTEINS
FORMING
FIBRILS |
||||||
| Transthyretin in amyloid fibrils | 1RVS |
synthetic | MAS |
Jaroniec
et al. (2004) |
||
| Amyloid
protofilaments of beta2-microglobulin fragment |
2E8D |
10 kDa/4 | Escherichia coli | MAS |
Iwata et
al. (2006) |
|
| WW domain of CA 150 |
2NNT |
18 kDa/4 | Escherichia coli | MAS |
Ferguson et
al. (2006) |
|
| Amyloid fibrils of the HET-s (218-289) prion |
2RNM |
43 kDa/5 | Escherichia coli | MAS |
Wasmer
et al. (2008) |
|
| MEMBRANE PROTEINS IN MEMBRANES |
||||||
|
Gramicidin |
synthetic | oriented
|
Ketchem et al. (1993) | |||
AchR M2 channel |
Escherichia coli | oriented
|
Opella et al. (1999) | |||
|
a-factor receptor M6 |
synthetic | oriented
|
Valentine et al. (2001) | |||
|
Influenza M2 channel residues 22-46 |
synthetic | oriented
|
Wang et al. (2001) | |||
|
2H95 | 8 kDa/4 | synthetic | oriented |
Hu et al. (2007) | |
|
synthetic | Cady et al. (2009) and Cady et al. (2010) |
||||
|
fd coat protein |
Enterobacteria phage fd | oriented |
Marassi and Opella (2003) | |||
|
HIV Vpu channel |
Escherichia coli | oriented
|
Park et al. (2003) | |||
human Phospholamban |
2KB7 | 30 kDa/5 | Escherichia coli | solution and oriented |
Traaseth et al. (2009) | |
Balayssac, S., Bertini, I., Bhaumik, A., Lelli, M., Luchinat, C.
(2008) Paramagnetic shifts in solid-state NMR of proteins to elicit
structural information Proc. Natl. Acad. Sci. Usa 105:17284-17289
Bertini I., Bhaumik A., De Paëpe G., Griffin R.G., Lelli M., Lewandowski J.R., Luchinat C. (2010) High-resolution solid-state NMR structure of a 17.6 kDa protein J. Am. Chem. Soc. 132:1032-1040.
Cady, S.D., Mishanina, T.V., Hong, M. (2009) Structure of amantadine-bound M2 transmembrane peptide of influenza A in lipid bilayers from magic-angle-spinning solid-state NMR: the role of Ser31 in amantadine binding J. Mol. Biol. 385:1127-1141
Cady, S.D., Schmidt-Rohr K., Wang J., Soto C.S., Degrado W.F., Hong,
M. (2010) Structure of the amantadine binding site of influenza M2
proton channels in lipid bilayers Nature 463:689-692
Castellani, F., Van Rossum, B., Diehl, A., Schubert, M., Rehbein, K., Oschkinat, H. (2002) Structure of a protein determined by solid-state magic-angle-spinning NMR spectroscopy Nature 420:98-102
Ferguson, N., Becker, J., Tidow, H., Tremmel, S., Sharpe, T.D., Krause, G., Flinders, J., Petrovich, M., Berriman, J., Oschkinat, H., Fersht, A.R. (2006) General structural motifs of amyloid protofilaments. Proc. Natl. Acad. Sci.Usa 103:16248-16253
Franks, W.T., Wylie, B.J., Schmidt, H.L., Nieuwkoop, A.J., Mayrhofer, R.M., Shah, G.J., Graesser, D.T., Rienstra, C.M. (2008) Dipole tensor-based atomic-resolution structure determination of a nanocrystalline protein by solid-state NMR Proc. Natl. Acad. Sci.Usa 105:4621-4626
Hu, J., Asbury, T., Achuthan, S., Li, C., Bertram, R., Quine, J.R., Fu, R., Cross, T.A. (2007) Backbone structure of the amantadine-blocked trans-membrane domain m2 proton channel from influenza a virus Biophys. J. 92:4335-4343
Iwata, K., Fujiwara, T., Matsuki, Y., Akutsu, H., Takahashi, S., Naiki, H., Goto, Y. (2006) 3D structure of amyloid protofilaments of beta2-microglobulin fragment probed by solid-state NMR Proc. Natl. Acad. Sci.Usa 103:18119-18124
Jaroniec, C.P., MacPhee, C.E., Bajaj, V.S., McMahon, M.T., Dobson, C.M., Griffin, R.G. (2004) High-resolution molecular structure of a peptide in an amyloid fibril determined by magic angle spinning NMR spectroscopy Proc. Natl. Acad. Sci.Usa 101:711-716
Ketchem, R.R., W. Hu, and T.A. Cross (1993) High-resolution conformation of gramicidin A in a lipid bilayer by solid-state NMR. Science 261:1457-1460
Lange, A., Becker, S., Seidel, K., Giller, K., Pongs, O., Baldus, M. (2005) A Concept for Rapid Protein-Structure Determination by Solid-State NMR Spectroscopy Angew. Chem. Int. Ed. Engl. 44:2089-2092
Loquet, A., Bardiaux, B., Gardiennet, C., Blanchet, C., Baldus, M., Nilges, M., Malliavin, T., Bockmann, A. (2008) 3D structure determination of the Crh protein from highly ambiguous solid-state NMR restraints. J. Am. Chem. Soc. 130:3579-3589
Manolikas, T., Herrmann, T., Meier, B.H. (2008) Protein structure determination from 13C spin-diffusion solid-state NMR spectroscopy. J. Am. Chem. Soc. 130:3959-3966
Marassi, FM, & Opella, SJ (2003) Simultaneous assignment and structure determination of a membrane protein from NMR orientational restraints. Protein Sci. 12:403-411
Opella, S.J., F.M. Marassi, J.J. Gesell, A.P. Valente, Y. Kim, M. Oblatt-Montal, and M. Montal (1999) Structures of the M2 channel-lining segments from nicotinic acetylcholine and NMDA receptors by NMR spectroscopy. Nat. Struct. Biol. 6:374-379
Park, S.H., A.A. Mrse, A.A. Nevzorov, M.F. Mesleh, M. Oblatt-Montal, M. Montal, and S.J. Opella (2003) Three-dimensional structure of the channel-forming trans-membrane domain of virus protein "u" (Vpu) from HIV-1. J. Mol. Biol. 333:409-424
Traaseth, N.J., Shi, L., Verardi, R., Mullen, D.G., Barany, G., Veglia, G. (2009) Structure and topology of monomeric phospholamban in lipid membranes determined by a hybrid solution and solid-state NMR approach. Proc. Natl. Acad. Sci.USA 106:10165-10170
Valentine, K.G., S.F. Liu, F.M. Marassi, G. Veglia, S.J. Opella, F.X. Ding, S.H. Wang, B. Arshava, J.M. Becker, and F. Naider (2001) Structure and topology of a peptide segment of the 6th transmembrane domain of the Saccharomyces cerevisae alpha-factor receptor in phospholipid bilayers. Biopolymers 59:243-256
Wang, J., S. Kim, F. Kovacs, and T.A. Cross (2001) Structure of the transmembrane region of the M2 protein H(+) channel. Protein Sci 10:2241-2250
Wasmer, C., Lange, A., Van Melckebeke, H., Siemer, A.B., Riek, R.,
Meier, B.H. (2008) Amyloid fibrils of the HET-s(218-289) prion form a
beta solenoid with a triangular hydrophobic core Science 319:1523-1526
Zhang Y., Doherty T., Li J., Lu W., Barinka C., Lubkowski J., Hong M. (2010) Resonance Assignment and Three-Dimensional Structure Determination of a Human a-Defensin, HNP-1, by solid-state NMR J. Mol. Biol.
Zhou, D.H., Shea, J.J., Nieuwkoop, A.J., Franks, W.T., Wylie, B.J., Mullen, C., Sandoz, D., Rienstra, C.M. (2007) Solid-State Protein-Structure Determination with Proton-Detected Triple-Resonance 3D Magic-Angle-Spinning NMR Spectroscopy. Angew. Chem. Int. Ed. Engl. 46:8380-8383