The table below provides information about proteins whose structures have been determined by solid-state NMR, to a resolution sufficient to have resulted in a file deposited at the RCSB Protein Data Bank.

Last database update : 23 february 2010

Unique proteins in database : 18

Number of coordinate files in database : 23

For more membrane protein structures: determined by NMR

 
Protein

PDB

Entry

BMRB

Entry

Oligomer

Size/State

Production

Source

NMR

Method

PubMed

Reference

MICRO-CRYSTALLINE PROTEINS
Spectrin SH3 domain
1M8M

7 kDa/1 Escherichia coli
MAS
Castellani et al. (2002)
Kaliotoxin

1XSW

4 kDa/1 Escherichia coli
MAS

Lange et al. (2005)

beta1 immunoglobulin binding domain of protein G (GB1)

2JU6

2JSV

6 kDa/1 Escherichia coli
MAS

Zhou et al. (2007) and

Franks et al. (2008)

Crh domain, swapped dimer 2RLZ
19 kDa/2 Escherichia coli
MAS
Loquet et al. (2008)
Ubiquitin
2JZZ

9 kDa/1 Escherichia coli
MAS
Manolikas et al. (2008)
MMP-12 (Macrophage metalloelastase)
2K9C
2KRJ
17 kDa/1 Escherichia coli
MAS
Balayssac et al. (2008) and
Bertini et al. (2010)
human alpha-defensin HNP-1
3 kDa/1
Escherichia coliMAS
Zhang et al. (2009)
 
PROTEINS FORMING FIBRILS
Transthyretin in amyloid fibrils 1RVS

1 kDa/1
synthetic
MAS
Jaroniec et al. (2004)
Amyloid protofilaments of beta2-microglobulin fragment
2E8D
10 kDa/4 Escherichia coli
MAS
Iwata et al. (2006)
WW domain of CA 150
2NNT

18 kDa/4 Escherichia coli
MAS
Ferguson et al. (2006)
Amyloid fibrils of the HET-s (218-289) prion
2RNM
43 kDa/5 Escherichia coli
MAS
Wasmer et al. (2008)
 
MEMBRANE PROTEINS IN MEMBRANES
Gramicidin

4 kDa/2
synthetic
oriented
Ketchem et al. (1993)
AchR M2 channel

3 kDa/1
Escherichia coli
oriented
Opella et al. (1999)
a-factor receptor M6

2 kDa/1
synthetic
oriented
Valentine et al. (2001)
Influenza M2 channel
residues 22-46

11 kDa/4
synthetic
oriented
Wang et al. (2001)

Influenza M2 channel
residues 22-46
with amantadine

2H95   8 kDa/4 synthetic
oriented
Hu et al. (2007)

Influenza M2 channel
residues 22-46
with amantadine

11 kDa/4
synthetic
MAS
Cady et al. (2009) and
Cady et al. (2010)
fd coat protein
5 kDa/1
Enterobacteria phage fd
oriented
Marassi and Opella (2003)
HIV Vpu channel

4 kDa/1
Escherichia coli
oriented
Park et al. (2003)

human Phospholamban

2KB7   30 kDa/5 Escherichia coli
solution and oriented
Traaseth et al. (2009)

References

Balayssac, S., Bertini, I., Bhaumik, A., Lelli, M., Luchinat, C. (2008) Paramagnetic shifts in solid-state NMR of proteins to elicit structural information Proc. Natl. Acad. Sci. Usa 105:17284-17289

Bertini I., Bhaumik A., De Paëpe G., Griffin R.G., Lelli M., Lewandowski J.R., Luchinat C. (2010) High-resolution solid-state NMR structure of a 17.6 kDa protein J. Am. Chem. Soc. 132:1032-1040.

Cady, S.D., Mishanina, T.V., Hong, M. (2009) Structure of amantadine-bound M2 transmembrane peptide of influenza A in lipid bilayers from magic-angle-spinning solid-state NMR: the role of Ser31 in amantadine binding J. Mol. Biol. 385:1127-1141

Cady, S.D., Schmidt-Rohr K., Wang J., Soto C.S., Degrado W.F., Hong, M. (2010) Structure of the amantadine binding site of influenza M2 proton channels in lipid bilayers Nature 463:689-692

Castellani, F., Van Rossum, B., Diehl, A., Schubert, M., Rehbein, K., Oschkinat, H. (2002) Structure of a protein determined by solid-state magic-angle-spinning NMR spectroscopy Nature 420:98-102

Ferguson, N., Becker, J., Tidow, H., Tremmel, S., Sharpe, T.D., Krause, G., Flinders, J., Petrovich, M., Berriman, J., Oschkinat, H., Fersht, A.R. (2006) General structural motifs of amyloid protofilaments. Proc. Natl. Acad. Sci.Usa 103:16248-16253

Franks, W.T., Wylie, B.J., Schmidt, H.L., Nieuwkoop, A.J., Mayrhofer, R.M., Shah, G.J., Graesser, D.T., Rienstra, C.M. (2008) Dipole tensor-based atomic-resolution structure determination of a nanocrystalline protein by solid-state NMR Proc. Natl. Acad. Sci.Usa 105:4621-4626

Hu, J., Asbury, T., Achuthan, S., Li, C., Bertram, R., Quine, J.R., Fu, R., Cross, T.A. (2007) Backbone structure of the amantadine-blocked trans-membrane domain m2 proton channel from influenza a virus Biophys. J. 92:4335-4343

Iwata, K., Fujiwara, T., Matsuki, Y., Akutsu, H., Takahashi, S., Naiki, H., Goto, Y. (2006) 3D structure of amyloid protofilaments of beta2-microglobulin fragment probed by solid-state NMR Proc. Natl. Acad. Sci.Usa 103:18119-18124

Jaroniec, C.P., MacPhee, C.E., Bajaj, V.S., McMahon, M.T., Dobson, C.M., Griffin, R.G. (2004) High-resolution molecular structure of a peptide in an amyloid fibril determined by magic angle spinning NMR spectroscopy Proc. Natl. Acad. Sci.Usa 101:711-716

Ketchem, R.R., W. Hu, and T.A. Cross (1993) High-resolution conformation of gramicidin A in a lipid bilayer by solid-state NMR. Science 261:1457-1460

Lange, A., Becker, S., Seidel, K., Giller, K., Pongs, O., Baldus, M. (2005) A Concept for Rapid Protein-Structure Determination by Solid-State NMR Spectroscopy Angew. Chem. Int. Ed. Engl. 44:2089-2092

Loquet, A., Bardiaux, B., Gardiennet, C., Blanchet, C., Baldus, M., Nilges, M., Malliavin, T., Bockmann, A. (2008) 3D structure determination of the Crh protein from highly ambiguous solid-state NMR restraints. J. Am. Chem. Soc. 130:3579-3589

Manolikas, T., Herrmann, T., Meier, B.H. (2008) Protein structure determination from 13C spin-diffusion solid-state NMR spectroscopy. J. Am. Chem. Soc. 130:3959-3966

Marassi, FM, & Opella, SJ (2003) Simultaneous assignment and structure determination of a membrane protein from NMR orientational restraints. Protein Sci. 12:403-411

Opella, S.J., F.M. Marassi, J.J. Gesell, A.P. Valente, Y. Kim, M. Oblatt-Montal, and M. Montal (1999) Structures of the M2 channel-lining segments from nicotinic acetylcholine and NMDA receptors by NMR spectroscopy. Nat. Struct. Biol. 6:374-379

Park, S.H., A.A. Mrse, A.A. Nevzorov, M.F. Mesleh, M. Oblatt-Montal, M. Montal, and S.J. Opella (2003) Three-dimensional structure of the channel-forming trans-membrane domain of virus protein "u" (Vpu) from HIV-1. J. Mol. Biol. 333:409-424

Traaseth, N.J., Shi, L., Verardi, R., Mullen, D.G., Barany, G., Veglia, G. (2009) Structure and topology of monomeric phospholamban in lipid membranes determined by a hybrid solution and solid-state NMR approach. Proc. Natl. Acad. Sci.USA 106:10165-10170

Valentine, K.G., S.F. Liu, F.M. Marassi, G. Veglia, S.J. Opella, F.X. Ding, S.H. Wang, B. Arshava, J.M. Becker, and F. Naider (2001) Structure and topology of a peptide segment of the 6th transmembrane domain of the Saccharomyces cerevisae alpha-factor receptor in phospholipid bilayers. Biopolymers 59:243-256

Wang, J., S. Kim, F. Kovacs, and T.A. Cross (2001) Structure of the transmembrane region of the M2 protein H(+) channel. Protein Sci 10:2241-2250

Wasmer, C., Lange, A., Van Melckebeke, H., Siemer, A.B., Riek, R., Meier, B.H. (2008) Amyloid fibrils of the HET-s(218-289) prion form a beta solenoid with a triangular hydrophobic core Science 319:1523-1526

Zhang Y., Doherty T., Li J., Lu W., Barinka C., Lubkowski J., Hong M. (2010) Resonance Assignment and Three-Dimensional Structure Determination of a Human a-Defensin, HNP-1, by solid-state NMR J. Mol. Biol.

Zhou, D.H., Shea, J.J., Nieuwkoop, A.J., Franks, W.T., Wylie, B.J., Mullen, C., Sandoz, D., Rienstra, C.M. (2007) Solid-State Protein-Structure Determination with Proton-Detected Triple-Resonance 3D Magic-Angle-Spinning NMR Spectroscopy. Angew. Chem. Int. Ed. Engl. 46:8380-8383