Dror Emmanuel WARSCHAWSKI
Born May 25th, 1969
RESEARCH EXPERIENCE
Institut de
Biologie Physico-Chimique, Paris (France)
Since 1998 Research associate at the Laboratoire de Biologie
Physico-Chimique des Protéines Membranaires, CNRS - UMR
7099. Advisor: Bruno Miroux.
Université du Québec à
Montréal, Québec (Canada)
2015-2016 Associate professor. "Solid-state NMR of intact and living cells", Sabbatical, 1 year. Advisor:
Isabelle Marcotte
2007 "Biophysical study of bicelles: NMR, atomic force microscopy,
mass spectrometry, infra-red spectroscopy", Sabbatical, 1 year. Advisor:
Isabelle Marcotte
Guelph University,
Ontario (Canada)
1997 "Structural study of membrane peptides by solid-state NMR",
Post-Doctorate, 3 months. Advisor: James H. Davis
Massachusetts Institute of
Technology, Cambridge (USA)
95-97 "Lipids and membrane peptides under
physiological conditions: structural and dynamical studies using
solid-state
NMR", Post-doctorate: 2 years. Advisor: Professor
Robert G. Griffin
Université Paris Diderot,
Paris (France)
92-95 "Biomembranes, model membranes: new approaches using
magic-angle
spinning solid-state NMR", Ph. D. in Molecular Biophysics ,
3 years. Advisor: Professor Philippe F. Devaux
Guelph University,
Ontario (Canada)
1992 "Lipid studies by solid-state NMR", 3 months. Advisor:
Professor James H. Davis
Institut de Biologie
Physico-Chimique, Paris (France)
1991 "Red blood cell study by EPR", 3 months. Advisor:
Doctor Alain Zachowski
Institut Curie, Paris
(France)
1990 "Study of an ion-plasma collision", 2 months
TEACHING EXPERIENCE
On the internet
I
manage the mailing list of NMR laboratories around the world, as well
as the two databases for protein structures determined by NMR:
since 2009 Teaching biological NMR for the MSc "Protein engineering
and industrial applications"
Canada
2016 Occasional teaching solid-state NMR at UQAM (Montréal) and Université Laval (Québec)
2006-2014 Teaching solid-state NMR for the MSc "Molecular and
Cellular Biophysics"
2005-2014 Teaching biological NMR for the MSc "Structure,
Proteomics and
Functional Genetics"
2001-2014 Teaching practical NMR for the MSc in Biology
Université
Paris-Sud 11
2006 Teaching biological solid-state NMR for the MSc "Structural and
Functional Engineering of Biomolecules"
Université Paris
Descartes
2004-2006 Member of the commission de spécialistes 31-32ème section
2001-2014 Teaching solid-state NMR for the MSc "Chemical and
Biological Analyses"
Université Paris
XIII Nord
2002-2009 Member of the commission de spécialistes 28ème section
Ecole Normale Supérieure,
Paris
2006-2009 Teaching biological solid-state NMR for the MSc in Biology
2001 Teaching solid-state NMR for professors of "classes
préparatoires aux grandes écoles"
Institut de Biologie
Physico-Chimique, Paris
since 1998 Tutoring post-docs, PhD, MSc and BSc students
EDUCATION AND HONORS
Université Paris
Diderot
2006 Habilitation to Direct Researches, Physics
92-95 Recipient of a fellowship from the Ministère de l'Enseignement
Supérieur et de la Recherche for a Ph. D. in Molecular Biophysics, Cum
Magna Laude. Advisor: Philippe F. Devaux
91-92 DEA de Biophysique Moléculaire and Magistère de Physique, Honors
AB
90-91 Maitrise de Physique (Masters in Physics), Honors AB
BIBLIOGRAPHY
M. Beaugrand, A. A. Arnold, A. Juneau, A. Balieiro
Gambaro, D. E. Warschawski, P. T. F. Williamson and I. Marcotte
"Magnetically-oriented bicelles with monoalkylphosphocholines –
versatile membrane mimetics for Nuclear Magnetic Resonance
applications". Langmuir 32:13244-13251 (2016)
X. L. Warnet, A. A. Arnold, I. Marcotte and D. E. Warschawski "NMR can now scrutinize the membrane of a living cell" Atlas of Science, 16 June (2016)
X. L. Warnet, M. Laadhari, A. A. Arnold, I. Marcotte
and D. E.
Warschawski "A 2H magic-angle
spinning solid-state NMR characterisation of lipid membranes in intact
bacteria" Biochim. Biophys.
Acta. 1858:146-152
(2016)
X. L. Warnet, A. A. Arnold, I. Marcotte
and D. E.
Warschawski "In-Cell Solid-State NMR: An Emerging Technique for the Study of Biological Membranes" Biophys. J. 109:2461–2466
(2015)
G.
Hattab, D. E. Warschawski, K. Moncoq and B. Miroux "Escherichia coli as
host for membrane protein structure determination: a global analysis" Sci. Rep. 5:12097 (2015)
A. A. Arnold, B. Genard, F. Zito, R. Tremblay, D. E.
Warschawski
and I. Marcotte "Identification of
lipid and saccharide constituents of whole microalgal cells by 13C
solid-state NMR" Biochim.
Biophys. Acta 1848:369-377
(2015)
G. Hattab, A. Y. T. Suisse, O. Ilioaia, M. Casiraghi,
M. Dezi, X. L.
Warnet, D. E. Warschawski, K. Moncoq, M. Zoonens and B. Miroux
"Membrane Protein Production in Escherichia coli: Overview and
Protocols" In Membrane Proteins Production for
Structural Analysis. I. Mus-Veteau (Ed.), Springer, New-York,
USA. Chapter 4, pp. 87-106
(2014)
L. J. Catoire, X. L. Warnet and D. E. Warschawski
"Micelles,
Bicelles, Amphipols, Nanodiscs, Liposomes, or Intact Cells: The
Hitchhiker’s Guide to the Study of Membrane Proteins by NMR" In Membrane Proteins Production for
Structural Analysis. I. Mus-Veteau (Ed.), Springer, New-York,
USA. Chapter 12, pp. 315-345
(2014)
M. Beaugrand, A. A. Arnold, J. Hénin, D. E. Warschawski, P. T. F.
Williamson, and I. Marcotte "Lipid
Concentration and Molar Ratio Boundaries for the Use of Isotropic
Bicelles" Langmuir 30:6162-6170 (2014)
D. Warschawski "La fin du monde: la faute à qui?" Le Courrier (Genève), mardi 6 mai, page 4 (2014)
A. A. Arnold, B. Genard, A. L. D. A. G. Da Silva, R. Tremblay, D. E.
Warschawski, F. Zito and I. Marcotte "Screening the Dynamics of
Membrane Constituents in Intact Microalgal Cells by Solid-State NMR"
Biophys. J. 106:508a (2014)
G.
Hattab, K. Moncoq, D. Warschawski and B. Miroux "Escherichia Coli as
Host for Membrane Protein Structure Determination: A Global Analysis" Biophys. J. 106:46a (2014)
A. Abdine,
K.-H. Park and D. E. Warschawski "Cell-free membrane protein expression
for solid-state NMR" Methods Mol. Biol. 831:85-109 (2012)
D. E. Warschawski, A. A. Arnold, M. Beaugrand, A.
Gravel, E.
Chartrand and I. Marcotte "Choosing
membrane mimetics for NMR structural studies of transmembrane proteins" Biochim.
Biophys. Acta 1808:1957-1974
(2011)
A.
Abdine, M. A. Verhoeven and D. E. Warschawski "Cell-free expression and
labeling strategies for a new decade in solid-state NMR" New Biotechnol. 28:272-276 (2011)
A.
Abdine, M. A. Verhoeven, K.-H. Park, A. Ghazi, E. Guittet, C.
Berrier, C. Van Heijenoort and D. E. Warschawski "Structural
study of the membrane protein MscL using cell-free expression and
solid-state NMR" J. Magn. Reson. 204:155-159 (2010)
M. N.
Triba,
P. F. Devaux and D. E. Warschawski "Effects of lipid chain length and
unsaturation on bicelles stability. A phosphorus NMR study" Biophys.
J. 91:1357-1367 (2006)
M. N.
Triba, M.
Zoonens, J.-L. Popot, P. F. Devaux and D. E. Warschawski
"Reconstitution and alignment by a magnetic field of a β-barrel
membrane protein in bicelles" Eur. Biophys. J. 35:268-275
(2006)
D.
E.
Warschawski and P. F. Devaux "1H-13C polarization transfer in
membranes: A tool for probing lipid dynamics and the effect of
cholesterol" J. Magn. Reson. 177:166-171 (2005)
D.
E.
Warschawski and P. F. Devaux "Order parameters of unsaturated
phospholipids in membranes and the effect of cholesterol: a 1H-13C
solid-state NMR study at natural abundance" Eur. Biophys. J. 34:987-996
(2005)
M.
N.
Triba, D. E. Warschawski and P. F. Devaux "Bicelles: disks or
perforated lamellae?" Eur. Biophys. J. 34:710 (2005)
O.
Wattraint, D. E. Warschawski and C. Sarazin "Tethered or adsorbed
supported lipid bilayers in nanotubes characterized by deuterium magic
angle spinning NMR spectroscopy" Langmuir 21:3226-3228
(2005)
M.N.
Triba, D.
E. Warschawski and P. F. Devaux "Reinvestigation by phosphorus NMR of
lipid distribution in bicelles" Biophys. J. 88:1887–1901
(2005)
M. N.
Triba, M.
Traïkia, D. E. Warschawski, L. Nicolas-Morgantini, A. Lety, P. Gilard
and P. F. Devaux "Proton Magic Angle Spinning-NMR Investigation of
Surfactant Aqueous Suspensions" Journal of Colloid and Interface
Science 274:341-345 (2004)
J.-L.
Popot, E. A.
Berry, D. Charvolin, C. Creuzenet, C. Ebel, D. M. Engelman, M.
Flötenmeyer, F. Giusti, Y. Gohon, P. Hervé, Q. Hong, J. H. Lakey, K.
Leonard, H. A. Shuman, P. Timmins, D. E. Warschawski, F. Zito, M.
Zoonens, B. Pucci and C.Tribet "Amphipols: polymeric surfactants for
membrane biology research" Cell. Mol. Life Sci. 60:1559-1574
(2003)
D.
E.
Warschawski and P. F. Devaux "Using DROSS to study the order of
unsaturated lipid, sphingolipids and lipid / cholesterol interactions" Eur.
Biophs. J. 32:288 (2003)
F.
Ferrage,
M. Zoonens, D. E. Warschawski, J.-L. Popot and G. Bodenhausen "Slow
Diffusion of Macromolecular Assemblies Measured by a New Pulsed Field
Gradient NMR Method" J. Am. Chem. Soc. 125:2541-2545
(2003)
Correction: J. Am.
Chem. Soc. 126:5654
(2004)
M.
Traïkia,
D. E. Warschawski, O. Lambert, J.-L. Rigaud and P. F. Devaux
"Asymmetrical Membranes and Surface Tension" Biophys. J. 83:1443-1454
(2002)
N.
Tsapis, R.
Ober,
A. Chaffotte, D. E. Warschawski, J. Everett, J. Kauffman, P. Kahn, M.
Waks
and W. Urbach "Modification of the elastic constants of a
peptide-decorated
lamellar phase", Langmuir 18:4384-4392 (2002)
D.
E.
Warschawski
and P.F. Devaux "Polarisation transfer in lipid membranes" J.
Magn. Resonance 145:367-372 (2000)
M.
Traikia,
D.
E. Warschawski, M. Recouvreur, J. Cartaud and P. F. Devaux "Formation
of unilameller vesicles by repetitive freeze-thaw cycles:
characterization
by electron microscopy and 31P-NMR" Eur. Biophs. J. 29:184-195
(2000)
D.
E.
Warschawski,
M. Traikia, P. F. Devaux and G. Bodenhausen, "Solid-State NMR for the
study of membrane systems: The use of anisotropic interactions"
Biochimie 80:437-450 (1998)
D.
E.
Warschawski,
J. D. Gross and R. G. Griffin, "Effects of membrane peptide dynamics
on high-resolution magic-angle spinning NMR" Journal de Chimie
Physique
95:460-466 (1998)
D.
E.
Warschawski, J. D. Gross and R. G. Griffin, "Dynamics of gramicidin A
deduced from high-resolution magic-angle spinning NMR" Biophys. J.
72:A325 (1997)
J. D.
Gross, D.
E. Warschawski and R. G. Griffin, "Dipolar recoupling in MAS NMR: A
probe for segmental order in lipid bilayers" J. Am. Chem. Soc. 119:796-802
(1997)
D.
E.
Warschawski,
P. Fellmann and P. F. Devaux, "High resolution 31P-1H two-dimensional
Nuclear Magnetic Resonance spectra of unsonicated lipid mixtures
spinning
at the magic-angle" Eur. Biophys. J. 25:131-137
(1996)
J. D.
Gross, P.
R. Costa, J.-P. Dubacq, D. E. Warschawski, P.-N. Lirsac, P. F. Devaux
and
R. G. Griffin, "Multidimensional NMR in lipid systems. Coherence
transfer
through J couplings under MAS" J. Magn. Reson.
B106:187-190 (1995)
D.
E.
Warschawski
and P. F. Devaux, "Water suppression in 1H MASS NMR spectra of lipids
and biological membranes" J. Magn. Reson. B106:76-79
(1995)
CONFERENCES
Organisation of the Workshop on
NMR of Biological Solids in Paris (France, 2009, 2011, 2013)
REVIEWS
For: Biochemistry, Biophysical Journal, Colloid and Surfaces, CRAS
Chimie,
European Biophysics Journal, FEBS letters, Journal of Membrane
Biology, Magnetic Resonance in
Chemistry, Magnetic Resonance in Medicine...
SKILLS
French, English, Hebrew, Spanish: Fluent
Computer languages used: BASIC, PASCAL, UNIX and VMS
Synthesis and purification of membrane peptides
INTERESTS
Creation of a music-band and public performances
between
1984 and 1995
Traveling all over the world
Webmaster of several websites and mailing lists