Dror Emmanuel WARSCHAWSKI
Born May 25th, 1969
RESEARCH EXPERIENCE
Sorbonne Univsersité, Paris
Since 2020 Research associate at the Laboratoire Chimie Physique et Chimie du Vivant (ex LBM), CNRS 8228- UMR . Advisor: Rodolphe Vuilleumier
Université du Québec à Montréal, Québec (Canada)
2015-2019 Associate professor at the Laboratoire de RMN des systèmes biologiques complexes. "Solid-state NMR of intact and living cells", Sabbatical, 4 years. Advisor: Isabelle Marcotte
2007 Invited professor at the Laboratoire de RMN des systèmes biologiques complexes. "Biophysical study of bicelles: NMR, atomic force microscopy, mass spectrometry, infra-red spectroscopy", Sabbatical, 1 year. Advisor: Isabelle Marcotte
Institut de Biologie Physico-Chimique, Paris
1998-2015 Research associate at the Laboratoire de Biologie Physico-Chimique des Protéines Membranaires, CNRS - UMR 7099. Advisor: Bruno Miroux
Guelph University, Ontario (Canada)
1997 "Structural study of membrane peptides by solid-state NMR", Post-Doctorate, 3 months. Advisor: James H. Davis
Massachusetts Institute of Technology, Cambridge (USA)
95-97 "Lipids and membrane peptides under physiological conditions: structural and dynamical studies using solid-state NMR", Post-doctorate: 2 years. Advisor: Professor Robert G. Griffin
Université Paris Diderot, Paris (France)
92-95 "Biomembranes, model membranes: new approaches using magic-angle spinning solid-state NMR", Ph. D. in Molecular Biophysics , 3 years. Advisor: Professor Philippe F. Devaux
Guelph University, Ontario (Canada)
1992 "Lipid studies by solid-state NMR", 3 months. Advisor: Professor James H. Davis
Institut de Biologie Physico-Chimique, Paris (France)
1991 "Red blood cell study by EPR", 3 months. Advisor: Doctor Alain Zachowski
Institut Curie, Paris (France)
1990 "Study of an ion-plasma collision", 2 months
TEACHING EXPERIENCE
Internet
I manage the mailing list of NMR
laboratories around the world:
http://www.drorlist.com/nmr.html
http://www.drorlist.com/rmnlist.html
http://www.drorlist.com/nmrlist.html
2009-2015 and 2020-2021 Teaching biological NMR for the MSc "Protein engineering and industrial applications"
Canada
2016-2019 Occasional teaching solid-state NMR at Université de Montréal, UQAM (Montréal) and Université Laval (Québec)
2006-2014 and 2022-2024 Teaching solid-state NMR for the MSc "Molecular and Cellular Biophysics"
2005-2014 Teaching biological NMR for the MSc "Structure, Proteomics and Functional Genetics"
2001-2014 Teaching practical NMR for the MSc in Biology
2006 Teaching biological solid-state NMR for the MSc "Structural and Functional Engineering of Biomolecules"
2004-2006 Member of the commission de spécialistes 31-32ème section
2001-2014 Teaching solid-state NMR for the MSc "Chemical and Biological Analyses"
2002-2009 Member of the commission de spécialistes 28ème section
Ecole Normale Supérieure, Paris
2006-2009 Teaching biological solid-state NMR for the MSc in Biology
2001 Teaching solid-state NMR for professors of "classes préparatoires aux grandes écoles"
Institut de Biologie Physico-Chimique, Université du Québec à Montréal and Sorbonne Université, Paris
since 1998 Tutoring post-docs, PhD, MSc and BSc students
EDUCATION AND HONORS
Université Paris Diderot
2006 Habilitation to Direct Researches, Physics
92-95 Recipient of a fellowship from the Ministère de l'Enseignement Supérieur et de la Recherche for a Ph. D. in Molecular Biophysics, Cum Magna Laude. Advisor: Philippe F. Devaux
91-92 DEA de Biophysique Moléculaire and Magistère de Physique, Honors AB
90-91 Maitrise de Physique (Masters in Physics), Honors AB
BIBLIOGRAPHY
Orcid : 0000-0002-4363-4965
Scopus : 6602213358
IDREF : 154796190
HAL : 887797
53) D. Veillard, S. Beauclercq, N. Ghafari, A. A. Arnold, B. Genard, L. Sleno, F. Olivier, A. Choquet, D. E. Warschawski, I. Marcotte and R. Tremblay "Molecular evidence of shipping noise impact on the blue mussel, a key species for the sustainability of coastal marine environments" Mar. Ecol. Prog. Ser. 759:35-50 (2025) ( https://hal.science/hal-05056432 ) https://doi.org/10.3354/meps14830
52) K. Kumar, A. A. Arnold, R. Gauthier, M. Mamone, J.-F. Paquin, D. E. Warschawski and I. Marcotte "Simultaneous assessment of membrane bilayer structure and drug insertion by 19F solid-state NMR". Biophys. J. 124:256–266 (2025) ( https://hal.science/hal-04911664 ) https://doi.org/10.1016/j.bpj.2024.11.3319
51) L. Zaatouf, K. Kumar, I. Marcotte and D. E. Warschawski "Assessment of membrane labelling mechanisms with exogenous fatty acids and detergents in bacteria". Biochimie 227B:12-18 (2024) ( https://hal.science/hal-04826831 ) https://doi.org/10.1016/j.biochi.2024.05.024
50) A. Poulhazan, A. A. Arnold, F. Mentink-Vigier, A. Muszyński, P. Azadi, A. Halim, S. Y. Vakhrushev, H. J. Joshi, T. Wang, D. E. Warschawski and I Marcotte "Molecular-level architecture of Chlamydomonas reinhardtii’s glycoprotein-rich cell wall" Nat. Commun. 15:986 (2024) ( https://hal.science/hal-04434789 ) https://doi.org/10.1038/s41467-024-45246-7
49)
K. Kumar, A. A. Arnold, R. Gauthier, M. Mamone, J.-F. Paquin, D. E.
Warschawski and I Marcotte "19F solid-state NMR approaches to probe
antimicrobial peptide interactions with membranes in whole cells"
Biochim. Biophys. Acta Biomembr. 1866:184269 (2024) ( https://hal.science/hal-04386127 ) https://doi.org/10.1016/j.bbamem.2023.184269
48) S. Beauclercq, O. Grenier, A. A. Arnold, D. E. Warschawski, G. H. Wikfors, B. Genard, R. Tremblay and I. Marcotte "Metabolomics and lipidomics reveal the effects of the toxic dinoflagellate Alexandrium catenella on immune cells of the blue mussel, Mytilus edulis" Harmful Algae 129:102529 (2023) (https://hal.science/hal-04268231 ) https://doi.org/10.1016/j.hal.2023.102529
47) K. Kumar, M. Sebastiao, A. A. Arnold, S. Bourgault, D. E. Warschawski and I. Marcotte "In situ solid-state NMR study of antimicrobial peptide interactions with erythrocyte membranes" Biophys. J. 121:1512-1524 (2022) (https://hal.science/hal-03656685 ) https://doi.org/10.1016/j.bpj.2022.03.009
46)
F. Laydevant, M. Mahabadi, P. Llido, J.-P. Bourgouin, L. Caron, A. A.
Arnold, I. Marcotte and D. E. Warschawski "Growth-phase dependence of
bacterial membrane lipid profile and labeling for in-cell solid-state
NMR applications" Biochim. Biophys. Acta 1864:183819
(2022) (https://hal.science/hal-03463942
) https://doi.org/10.1016/j.bbamem.2021.183819
45)
A. Poulhazan, M. C. Dickwella Widanage, A. Muszyński, A. A. Arnold, D.
E. Warschawski, P. Azadi, I. Marcotte and T. Wang "Identification and
Quantification of Glycans in Whole Cells: Architecture of Microalgal
Polysaccharides Described by Solid-State Nuclear Magnetic Resonance" J. Am. Chem. Soc. 143:19374-19388
(2021) (https://hal.science/hal-03419267v2)
https://doi.org/10.1021/jacs.1c07429
44) X.
Yan, K. Kumar, R. Miclette Lamarche, H. Youssef, G. S. Shaw, I.
Marcotte, C. E. DeWolf, D. E. Warschawski and E. Boisselier
"Interactions between the Cell Membrane Repair Protein S100A10 and
Phospholipid Monolayers and Bilayers" Langmuir 37:9652–9663
(2021) (https://hal.science/hal-03367662
) https://doi.org/10.1021/acs.langmuir.1c00342
43)
Z. Bouhlel, A. A. Arnold, J.-S. Deschênes, J.-L. Mouget, D. E.
Warschawski, R. Tremblay and I. Marcotte "Investigating the action of
the microalgal pigment marennine on Vibrio splendidus by in
vivo 2H and 31P solid-state NMR" Biochim. Biophys. Acta
Biomembr. 1863:183642 (2021)
(https://hal.science/hal-03242868 ) https://doi.org/10.1016/j.bbamem.2021.183642
42)
D. E.Warschawski, K. V. Lakshmi and I. Marcotte « Foreword to:
Biophysical studies of membrane systems and interactions - Commemorative
issue in honour of Professor Michèle Auger » Biochim. Biophys. Acta
Biomembr. 1863:183609 (2021) (https://hal.science/hal-03201135
) https://doi.org/10.1016/j.bbamem.2021.183609
41)
A. Poulhazan, A. A. Arnold, D. E. Warschawski and I. Marcotte
« Solid-state NMR study of microalgal membranes and cell
walls », in Solid-state NMR: Applications in
Biomembrane Structure (F. Separovic and M. A. Sani Eds.),
Institute of Physics Publishing, Bristol, UK. Chapter 4, pp. 1-23
(2020) (https://hal.science/hal-03089739
) https://doi.org/10.1088/978-0-7503-2532-5ch4
40) A. Poulhazan, A. A. Arnold, D. E. Warschawski and I. Marcotte "Unambiguous Ex Situ and in Cell 2D 13C Solid-State NMR Characterization of Starch and Its Constituents" in In-Cell NMR Spectroscopy. Biomolecular Structure and Function (A. Shekhtman and D. S. Burz Eds.), MDPI Books, Basel, Switzerland. Chapter 8, pp. 122-138 (2020) (https://hal.science/hal-02569401 ) https://doi.org/10.3390/books978-3-03928-255-5
39)
A. E. Gravel, A. A. Arnold, M. Fillion, M. Auger, D. E. Warschawski and
I. Marcotte " Magnetically-orientable Tween-based Model Membranes for
NMR Studies of Proteins" Biochim. Biophys. Acta Biomembr. 1862:183379
(2020) (https://hal.science/hal-02861563
) https://doi.org/10.1016/j.bbamem.2020.183379
38) X.
Yan, F. Noël, I. Marcotte, C. E. DeWolf, D. E. Warschawski and E.
Boisselier "AHNAK C-Terminal Peptide Membrane Binding-Interactions
Between the Residues 5654-5673 of AHNAK and Phospholipid Monolayers and
Bilayers" Langmuir 36:362-369 (2020) (https://hal.science/hal-02567660
) https://doi.org/10.1021/acs.langmuir.9b02973
Z. Bouhlel, D. E. Warschawski, A. A. Arnold, K. Lemarchand, R. Tremblay, and I. Marcotte "Solid-State NMR of Intact Bacteria Reveals the Effect of Stress and Antimicrobial Agents" Biophys. J. 118:344a (2020)(https://hal.science/hal-03031123 )
37)
Z. Bouhlel, A. A. Arnold, D. E. Warschawski, K. Lemarchand, R. Tremblay
and I. Marcotte "Labelling strategy and membrane characterization of
marine bacteria Vibrio splendidus by in vivo 2H NMR" Biochim.
Biophys. Acta 1861:871-878 (2019) (https://hal.science/hal-02567658
) https://doi.org/10.1016/j.bbamem.2019.01.018
36)
A. Poulhazan, A. A. Arnold, D. E. Warschawski and I. Marcotte
"Unambiguous Ex Situ and in Cell 2D 13C Solid-State NMR
Characterization of Starch and Its Constituents" Int. J. Mol. Sci.
19:3817 (2018) (https://hal.science/hal-02567657
) https://doi.org/10.3390/ijms19123817
35) E. Azar, D. Constantin
and D. E. Warschawski "The effect of gramicidin inclusions on the local
order of membrane components" Eur. Phys. J. E 41:44
(2018) (https://hal.science/hal-01744850
) https://doi.org/10.1140/epje/i2018-11644-5
34) D. E. Warschawski,
A. A. Arnold and I. Marcotte "A New Method of Assessing Lipid Mixtures
by 31P Magic-Angle Spinning NMR" Biophys. J. 114:1368–1376
(2018) (https://hal.science/hal-02567656
) https://doi.org/10.1016/j.bpj.2018.01.025
33)
A. A. Arnold, J.-P. Bourgouin,
B. Genard, D. E. Warschawski, R. Tremblay and I .Marcotte "Whole cell
solid-state NMR study of Chlamydomonas reinhardtii microalgae" J.
Biomol. NMR 70:123-131 (2018) (https://hal.science/hal-02567652
) https://doi.org/10.1007/s10858-018-0164-7
32) V. Booth, D. E.
Warschawski, N. P. Santisteban, M. Laadhari and I. Marcotte "Recent
progress on the application of 2H solid-state NMR to probe the
interaction of antimicrobial peptides with intact bacteria". Biochim. Biophys. Acta 1865:1500-1511
(2017) (https://hal.science/hal-02567650
) https://doi.org/10.1016/j.bbapap.2017.07.018
25) L. J. Catoire, X. L. Warnet and D. E. Warschawski "Micelles, Bicelles, Amphipols, Nanodiscs, Liposomes, or Intact Cells: The Hitchhiker’s Guide to the Study of Membrane Proteins by NMR" In Membrane Proteins Production for Structural Analysis. I. Mus-Veteau (Ed.), Springer, New-York, USA. Chapter 12, pp. 315-345 (2014) (https://hal.science/hal-02567627 ) https://doi.org/10.1007/978-1-4939-0662-8_12
24) M. Beaugrand, A. A. Arnold, J. Hénin, D. E. Warschawski, P. T. F. Williamson, and I. Marcotte "Lipid Concentration and Molar Ratio Boundaries for the Use of Isotropic Bicelles" Langmuir 30:6162-6170 (2014) (https://hal.science/hal-01498056 ) https://doi.org/10.1021/la5004353
21) A. Abdine, M. A. Verhoeven and D. E. Warschawski "Cell-free
expression and labeling strategies for a new decade in solid-state NMR"
New Biotechnol. 28:272-276
(2011) (https://hal.science/hal-02567621
)
https://doi.org/10.1016/j.nbt.2010.07.014
20) A.
Abdine, M. A. Verhoeven, K.-H. Park, A. Ghazi, E. Guittet, C. Berrier,
C. Van Heijenoort and D. E. Warschawski "Structural study of the
membrane protein MscL using cell-free expression and solid-state NMR" J. Magn. Reson. 204:155-159
(2010) (https://hal.science/hal-02567620
) https://doi.org/10.1016/j.jmr.2010.02.003
19) M. N.
Triba, P. F. Devaux and D. E. Warschawski "Effects of lipid chain length
and unsaturation on bicelles stability. A phosphorus NMR study" Biophys.
J. 91:1357-1367 (2006) (https://hal.science/hal-02567618
) https://doi.org/10.1529/biophysj.106.085118
18) M. N.
Triba, M. Zoonens, J.-L. Popot, P. F. Devaux and D. E. Warschawski
"Reconstitution and alignment by a magnetic field of a β-barrel membrane
protein in bicelles" Eur. Biophys. J. 35:268-275
(2006) (https://hal.science/hal-02567617
) https://doi.org/10.1007/s00249-005-0014-x
17)
D. E. Warschawski and P. F. Devaux "1H-13C polarization transfer in
membranes: A tool for probing lipid dynamics and the effect of
cholesterol" J. Magn. Reson. 177:166-171 (2005) (https://hal.science/hal-02567616 ) https://doi.org/10.1016/j.jmr.2005.07.011
16)
D. E. Warschawski and P. F. Devaux "Order parameters of unsaturated
phospholipids in membranes and the effect of cholesterol: a 1H-13C
solid-state NMR study at natural abundance" Eur. Biophys. J. 34:987-996
(2005) (https://hal.science/hal-02567615
) https://doi.org/10.1007/s00249-005-0482-z
15) O.
Wattraint, D. E. Warschawski and C. Sarazin "Tethered or adsorbed
supported lipid bilayers in nanotubes characterized by deuterium magic
angle spinning NMR spectroscopy" Langmuir 21:3226-3228
(2005) (https://hal.science/hal-02567614
) https://doi.org/10.1021/la0469147
14) M.N. Triba, D. E. Warschawski and P. F. Devaux "Reinvestigation by phosphorus NMR of lipid distribution in bicelles" Biophys. J. 88:1887–1901 (2005) (https://hal.science/hal-02567611 ) https://doi.org/10.1529/biophysj.104.055061
13) M. N.
Triba, M. Traïkia, D. E. Warschawski, L. Nicolas-Morgantini, A. Lety, P.
Gilard and P. F. Devaux "Proton Magic Angle Spinning-NMR Investigation
of Surfactant Aqueous Suspensions" Journal of Colloid and Interface
Science 274:341-345 (2004) (https://hal.science/hal-02567610
)
https://doi.org/10.1016/j.jcis.2004.01.013
11) F.
Ferrage, M. Zoonens, D. E. Warschawski, J.-L. Popot and G. Bodenhausen
"Slow Diffusion of Macromolecular Assemblies Measured by a New Pulsed
Field Gradient NMR Method" J. Am. Chem. Soc. 125:2541-2545
(2003) (https://hal.science/hal-02567606
) https://doi.org/10.1021/ja0211407
Correction: J. Am. Chem. Soc. 126:5654 (2004) (https://hal.science/hal-02567609 )
10) M. Traïkia, D. E. Warschawski, O. Lambert, J.-L. Rigaud and P. F. Devaux "Asymmetrical Membranes and Surface Tension" Biophys. J. 83:1443-1454 (2002) (https://hal.science/hal-02568215 ) https://doi.org/10.1016/s0006-3495(02)73915-59) N.
Tsapis, R. Ober, A. Chaffotte, D. E. Warschawski, J. Everett, J.
Kauffman, P. Kahn, M. Waks and W. Urbach "Modification of the elastic
constants of a peptide-decorated lamellar phase", Langmuir 18:4384-4392
(2002) (https://hal.science/hal-02567603
) https://doi.org/10.1021/la015745c
8) D.
E. Warschawski and P.F. Devaux "Polarisation transfer in lipid
membranes" J. Magn. Resonance 145:367-372 (2000) (https://hal.science/hal-02567602 ) https://doi.org/10.1006/jmre.2000.2135
7) M.
Traikia, D. E. Warschawski, M. Recouvreur, J. Cartaud and P. F. Devaux
"Formation of unilameller vesicles by repetitive freeze-thaw cycles:
characterization by electron microscopy and 31P-NMR" Eur. Biophs. J.
29:184-195 (2000) (https://hal.science/hal-02567600
) https://doi.org/10.1007/s002490000077
6) D.
E. Warschawski, M. Traikia, P. F. Devaux and G. Bodenhausen,
"Solid-State NMR for the study of membrane systems: The use of
anisotropic interactions" Biochimie 80:437-450
(1998) (https://hal.science/hal-02567597
)
https://doi.org/10.1016/s0300-9084(00)80011-5
5) D.
E. Warschawski, J. D. Gross and R. G. Griffin, "Effects of membrane
peptide dynamics on high-resolution magic-angle spinning NMR" Journal
de Chimie Physique 95:460-466 (1998) (https://hal.science/hal-02567599
) https://doi.org/10.1051/jcp:1998160
3) D. E. Warschawski, P. Fellmann and P. F. Devaux, "High resolution 31P-1H two-dimensional Nuclear Magnetic Resonance spectra of unsonicated lipid mixtures spinning at the magic-angle" Eur. Biophys. J. 25:131-137 (1996) (https://hal.science/hal-02567595 ) https://doi.org/10.1007/s002490050024
2) J. D.
Gross, P. R. Costa, J.-P. Dubacq, D. E. Warschawski, P.-N. Lirsac, P. F.
Devaux and R. G. Griffin, "Multidimensional NMR in lipid systems.
Coherence transfer through J couplings under MAS" J. Magn.
Reson. B106:187-190 (1995) (https://hal.science/hal-02567594
) https://doi.org/10.1006/jmrb.1995.1031
1) D. E. Warschawski and P. F. Devaux, "Water suppression in 1H MASS NMR spectra of lipids and biological membranes" J. Magn. Reson. B106:76-79 (1995) (https://hal.science/hal-02567592 ) https://doi.org/10.1006/jmrb.1995.1012
CONFERENCES
Organisation of the Workshop
on NMR of Biological Solids in Paris (France, 2009, 2011,
2013)
REVIEWS
For: BBA - Biomembranes, Biochemistry, Biophysical Journal, Colloid and
Surfaces, Communications Chemistry, CRAS Chimie, European Biophysics
Journal, FEBS letters, Journal of Membrane Biology, Journal
of Physical Chemistry, Langmuir, Magnetic Resonance in Chemistry,
Magnetic Resonance in Medicine, Phytochemical Analysis, Scientific
Reports, Solid-State NMR...
SKILLS
Computer languages used: BASIC, PASCAL, UNIX and VMS
Synthesis and purification of membrane peptides
INTERESTS
Traveling all over the world
Webmaster of several websites and mailing lists