CV in English

Dror Emmanuel WARSCHAWSKI

Born May 25th, 1969

RESEARCH EXPERIENCE

Sorbonne Univsersité, Paris

Since 2020 Research associate at the Laboratoire des Biomolécules, CNRS - UMR 7203. Advisor: Olivier Lequin

Université du Québec à Montréal, Québec (Canada)

2015-2019 Associate professor at the Laboratoire de RMN des systèmes biologiques complexes. "Solid-state NMR of intact and living cells", Sabbatical, 4 years. Advisor: Isabelle Marcotte

2007 Invited professor at the Laboratoire de RMN des systèmes biologiques complexes. "Biophysical study of bicelles: NMR, atomic force microscopy, mass spectrometry, infra-red spectroscopy", Sabbatical, 1 year. Advisor: Isabelle Marcotte

Institut de Biologie Physico-Chimique, Paris

1998-2015 Research associate at the Laboratoire de Biologie Physico-Chimique des Protéines Membranaires, CNRS - UMR 7099. Advisor: Bruno Miroux

Guelph University, Ontario (Canada)

1997 "Structural study of membrane peptides by solid-state NMR", Post-Doctorate, 3 months. Advisor: James H. Davis

Massachusetts Institute of Technology, Cambridge (USA)

95-97 "Lipids and membrane peptides under physiological conditions: structural and dynamical studies using solid-state NMR", Post-doctorate: 2 years. Advisor: Professor Robert G. Griffin

Université Paris Diderot, Paris (France)

92-95 "Biomembranes, model membranes: new approaches using magic-angle spinning solid-state NMR", Ph. D. in Molecular Biophysics , 3 years. Advisor: Professor Philippe F. Devaux

Guelph University, Ontario (Canada)

1992 "Lipid studies by solid-state NMR", 3 months. Advisor: Professor James H. Davis

Institut de Biologie Physico-Chimique, Paris (France)

1991 "Red blood cell study by EPR", 3 months. Advisor: Doctor Alain Zachowski

Institut Curie, Paris (France)

1990 "Study of an ion-plasma collision", 2 months

TEACHING EXPERIENCE

Internet

I manage the mailing list of NMR laboratories around the world:

http://www.drorlist.com/nmr.html
http://www.drorlist.com/rmnlist.html
http://www.drorlist.com/nmrlist.html

Agro Paris Tech

2009-2015 and since 2020 Teaching biological NMR for the MSc "Protein engineering and industrial applications"

Canada

2016-2019 Occasional teaching solid-state NMR at Université de Montréal, UQAM (Montréal) and Université Laval (Québec)

Université Paris Diderot

2006-2014 Teaching solid-state NMR for the MSc "Molecular and Cellular Biophysics"

2005-2014 Teaching biological NMR for the MSc "Structure, Proteomics and Functional Genetics"

2001-2014 Teaching practical NMR for the MSc in Biology

Université Paris-Sud 11

2006 Teaching biological solid-state NMR for the MSc "Structural and Functional Engineering of Biomolecules"

Université Paris Descartes

2004-2006 Member of the commission de spécialistes 31-32ème section

2001-2014 Teaching solid-state NMR for the MSc "Chemical and Biological Analyses"

Université Paris XIII Nord

2002-2009 Member of the commission de spécialistes 28ème section

Ecole Normale Supérieure, Paris

2006-2009 Teaching biological solid-state NMR for the MSc in Biology

2001 Teaching solid-state NMR for professors of "classes préparatoires aux grandes écoles"

Institut de Biologie Physico-Chimique, Paris

since 1998 Tutoring post-docs, PhD, MSc and BSc students

EDUCATION AND HONORS

Université Paris Diderot

2006 Habilitation to Direct Researches, Physics

92-95 Recipient of a fellowship from the Ministère de l'Enseignement Supérieur et de la Recherche for a Ph. D. in Molecular Biophysics, Cum Magna Laude. Advisor: Philippe F. Devaux

91-92 DEA de Biophysique Moléculaire and Magistère de Physique, Honors AB

90-91 Maitrise de Physique (Masters in Physics), Honors AB

BIBLIOGRAPHY

Research Gate

Orcid : 0000-0002-4363-4965

Scopus : 6602213358

IDREF : 154796190

HAL : 887797

47) K. Kumar, M. Sebastiao, A. A. Arnold, S. Bourgault, D. E. Warschawski and I. Marcotte "In situ solid-state NMR study of antimicrobial peptide interactions with erythrocyte membranes" Biophys. J. 121:1512-1524 (2022) https://doi.org/10.1016/j.bpj.2022.03.009

46) F. Laydevant, M. Mahabadi, P. Llido, J.-P. Bourgouin, L. Caron, A. A. Arnold, I. Marcotte and D. E. Warschawski "Growth-phase dependence of bacterial membrane lipid profile and labeling for in-cell solid-state NMR applications" Biochim. Biophys. Acta 1864:183819 (2022) https://doi.org/10.1016/j.bbamem.2021.183819

45) A. Poulhazan, M. C. Dickwella Widanage, A. Muszyński, A. A. Arnold, D. E. Warschawski, P. Azadi, I. Marcotte and T. Wang "Identification and Quantification of Glycans in Whole Cells: Architecture of Microalgal Polysaccharides Described by Solid-State Nuclear Magnetic Resonance" J. Am. Chem. Soc. 143:19374-19388 (2021) https://doi.org/10.1021/jacs.1c07429

44) X. Yan, K. Kumar, R. Miclette Lamarche, H. Youssef, G. S. Shaw, I. Marcotte, C. E. DeWolf, D. E. Warschawski and E. Boisselier "Interactions between the Cell Membrane Repair Protein S100A10 and Phospholipid Monolayers and Bilayers" Langmuir 37:9652–9663 (2021) https://doi.org/10.1021/acs.langmuir.1c00342

43) Z. Bouhlel, A. A. Arnold, J.-S. Deschênes, J.-L. Mouget, D. E. Warschawski, R. Tremblay and I. Marcotte "Investigating the action of the microalgal pigment marennine on Vibrio splendidus by in vivo 2H and 31P solid-state NMR" Biochim. Biophys. Acta Biomembr. 1863:183642 (2021) https://doi.org/10.1016/j.bbamem.2021.183642

42) D. E.Warschawski, K. V. Lakshmi and I. Marcotte « Foreword to: Biophysical studies of membrane systems and interactions - Commemorative issue in honour of Professor Michèle Auger » Biochim. Biophys. Acta Biomembr. 1863:183609 (2021) https://doi.org/10.1016/j.bbamem.2021.183609

41) A. Poulhazan, A. A. Arnold, D. E. Warschawski and I. Marcotte « Solid-state NMR study of microalgal membranes and cell walls », in Solid-state NMR: Applications in Biomembrane Structure (F. Separovic and M. A. Sani Eds.), Institute of Physics Publishing, Bristol, UK. Chapter 4, pp. 1-23 (2020) https://doi.org/10.1088/978-0-7503-2532-5ch4

40) A. Poulhazan, A. A. Arnold, D. E. Warschawski and I. Marcotte "Unambiguous Ex Situ and in Cell 2D ¹³C Solid-State NMR Characterization of Starch and Its Constituents" in In-Cell NMR Spectroscopy. Biomolecular Structure and Function (A. Shekhtman and D. S. Burz Eds.), MDPI Books, Basel, Switzerland. Chapter 8, pp. 122-138 (2020) https://doi.org/10.3390/books978-3-03928-255-5

39) A. E. Gravel, A. A. Arnold, M. Fillion, M. Auger, D. E. Warschawski and I. Marcotte " Magnetically-orientable Tween-based Model Membranes for NMR Studies of Proteins" Biochim. Biophys. Acta Biomembr. 1862:183379 (2020) https://doi.org/10.1016/j.bbamem.2020.183379

38) X. Yan, F. Noël, I. Marcotte, C. E. DeWolf, D. E. Warschawski and E. Boisselier "AHNAK C-Terminal Peptide Membrane Binding-Interactions Between the Residues 5654-5673 of AHNAK and Phospholipid Monolayers and Bilayers" Langmuir 36:362-369 (2020) https://doi.org/10.1021/acs.langmuir.9b02973

Z. Bouhlel, D. E. Warschawski, A. A. Arnold, K. Lemarchand, R. Tremblay, and I. Marcotte "Solid-State NMR of Intact Bacteria Reveals the Effect of Stress and Antimicrobial Agents" Biophys. J. 118:344a (2020)

37) Z. Bouhlel, A. A. Arnold, D. E. Warschawski, K. Lemarchand, R. Tremblay and I. Marcotte "Labelling strategy and membrane characterization of marine bacteria Vibrio splendidus by in vivo 2H NMR" Biochim. Biophys. Acta 1861:871-878 (2019) https://doi.org/10.1016/j.bbamem.2019.01.018

36) A. Poulhazan, A. A. Arnold, D. E. Warschawski and I. Marcotte "Unambiguous Ex Situ and in Cell 2D ¹³C Solid-State NMR Characterization of Starch and Its Constituents" Int. J. Mol. Sci. 19:3817 (2018) https://doi.org/10.3390/ijms19123817

35) E. Azar, D. Constantin and D. E. Warschawski "The effect of gramicidin inclusions on the local order of membrane components" Eur. Phys. J. E 41:44 (2018) https://doi.org/10.1140/epje/i2018-11644-5

34) D. E. Warschawski, A. A. Arnold and I. Marcotte "A New Method of Assessing Lipid Mixtures by 31P Magic-Angle Spinning NMR" Biophys. J. 114:1368–1376 (2018) https://doi.org/10.1016/j.bpj.2018.01.025

33) A. A. Arnold, J.-P. Bourgouin, B. Genard, D. E. Warschawski, R. Tremblay and I .Marcotte "Whole cell solid-state NMR study of Chlamydomonas reinhardtii microalgae" J. Biomol. NMR 70:123-131 (2018) https://doi.org/10.1007/s10858-018-0164-7

J.-P. Bourgouin, A. Poulhazan, F. Zito, A. A. Arnold, D. E. Warschawski, I. Marcotte « Methodological Development to Study Lipid Membranes of Intact Bacteria and Microalgae by 2H Solid-State NMR » Biophys. J. 114:159a (2018)

A. A. Arnold, J.-P. Bourgouin, B. Génard, D. E. Warschawski, F. Zito, R. Tremblay, I. Marcotte « Whole Cell 13C Solid-State NMR of a Fully Labelled Micro-organism: How Far Can We Go? » Biophys. J. 114:158a (2018)

A. E. Gravel, A. A. Arnold, D. E. Warschawski, I. Marcotte « Making Soft Magnetically-Orientable Membranes: An Alternative to Bicelles » Biophys. J. 114:95a (2018)

32) V. Booth, D. E. Warschawski, N. P. Santisteban, M. Laadhari and I. Marcotte "Recent progress on the application of 2H solid-state NMR to probe the interaction of antimicrobial peptides with intact bacteria". Biochim. Biophys. Acta 1865:1500-1511 (2017) https://doi.org/10.1016/j.bbapap.2017.07.018

31) M. Beaugrand, A. A. Arnold, A. Juneau, A. Balieiro Gambaro, D. E. Warschawski, P. T. F. Williamson and I. Marcotte "Magnetically-oriented bicelles with monoalkylphosphocholines – versatile membrane mimetics for Nuclear Magnetic Resonance applications". Langmuir 32:13244-13251 (2016) https://doi.org/10.1021/acs.langmuir.6b03099

X. L. Warnet, A. A. Arnold, I. Marcotte and D. E. Warschawski "NMR can now scrutinize the membrane of a living cell" Atlas of Science, 16 June (2016)

30) X. L. Warnet, M. Laadhari, A. A. Arnold, I. Marcotte and D. E. Warschawski "A 2H magic-angle spinning solid-state NMR characterisation of lipid membranes in intact bacteria" Biochim. Biophys. Acta. 1858:146-152 (2016) https://doi.org/10.1016/j.bbamem.2015.10.020

29) X. L. Warnet, A. A. Arnold, I. Marcotte and D. E. Warschawski "In-Cell Solid-State NMR: An Emerging Technique for the Study of Biological Membranes" Biophys. J. 109:2461–2466 (2015) https://doi.org/10.1016/j.bpj.2015.10.041

28) G. Hattab, D. E. Warschawski, K. Moncoq and B. Miroux "Escherichia coli as host for membrane protein structure determination: a global analysis" Sci. Rep. 5:12097 (2015) https://doi.org/10.1038/srep12097

27) A. A. Arnold, B. Genard, F. Zito, R. Tremblay, D. E. Warschawski and I. Marcotte "Identification of lipid and saccharide constituents of whole microalgal cells by 13C solid-state NMR" Biochim. Biophys. Acta 1848:369-377 (2015) https://doi.org/10.1016/j.bbamem.2014.07.017

26) G. Hattab, A. Y. T. Suisse, O. Ilioaia, M. Casiraghi, M. Dezi, X. L. Warnet, D. E. Warschawski, K. Moncoq, M. Zoonens and B. Miroux "Membrane Protein Production in Escherichia coli: Overview and Protocols" In Membrane Proteins Production for Structural Analysis. I. Mus-Veteau (Ed.), Springer, New-York, USA. Chapter 4, pp. 87-106 (2014) https://doi.org/10.1007/978-1-4939-0662-8_4

25) L. J. Catoire, X. L. Warnet and D. E. Warschawski "Micelles, Bicelles, Amphipols, Nanodiscs, Liposomes, or Intact Cells: The Hitchhiker’s Guide to the Study of Membrane Proteins by NMR" In Membrane Proteins Production for Structural Analysis. I. Mus-Veteau (Ed.), Springer, New-York, USA. Chapter 12, pp. 315-345 (2014) https://doi.org/10.1007/978-1-4939-0662-8_12

24) M. Beaugrand, A. A. Arnold, J. Hénin, D. E. Warschawski, P. T. F. Williamson, and I. Marcotte "Lipid Concentration and Molar Ratio Boundaries for the Use of Isotropic Bicelles" Langmuir 30:6162-6170 (2014) https://doi.org/10.1021/la5004353

D. Warschawski "La fin du monde: la faute à qui?" Le Courrier (Genève), mardi 6 mai, page 4 (2014)

A. A. Arnold, B. Genard, A. L. D. A. G. Da Silva, R. Tremblay, D. E. Warschawski, F. Zito and I. Marcotte "Screening the Dynamics of Membrane Constituents in Intact Microalgal Cells by Solid-State NMR" Biophys. J. 106:508a (2014)

G. Hattab, K. Moncoq, D. Warschawski and B. Miroux "Escherichia Coli as Host for Membrane Protein Structure Determination: A Global Analysis" Biophys. J. 106:46a (2014)

23) A. Abdine, K.-H. Park and D. E. Warschawski "Cell-free membrane protein expression for solid-state NMR" Methods Mol. Biol. 831:85-109 (2012) https://doi.org/10.1007/978-1-61779-480-3_6

22) D. E. Warschawski, A. A. Arnold, M. Beaugrand, A. Gravel, E. Chartrand and I. Marcotte "Choosing membrane mimetics for NMR structural studies of transmembrane proteins" Biochim. Biophys. Acta 1808:1957-1974 (2011) https://doi.org/10.1016/j.bbamem.2011.03.016

21) A. Abdine, M. A. Verhoeven and D. E. Warschawski "Cell-free expression and labeling strategies for a new decade in solid-state NMR" New Biotechnol. 28:272-276 (2011) https://doi.org/10.1016/j.nbt.2010.07.014

20) A. Abdine, M. A. Verhoeven, K.-H. Park, A. Ghazi, E. Guittet, C. Berrier, C. Van Heijenoort and D. E. Warschawski "Structural study of the membrane protein MscL using cell-free expression and solid-state NMR" J. Magn. Reson. 204:155-159 (2010) https://doi.org/10.1016/j.jmr.2010.02.003

19) M. N. Triba, P. F. Devaux and D. E. Warschawski "Effects of lipid chain length and unsaturation on bicelles stability. A phosphorus NMR study" Biophys. J. 91:1357-1367 (2006) https://doi.org/10.1529/biophysj.106.085118

18) M. N. Triba, M. Zoonens, J.-L. Popot, P. F. Devaux and D. E. Warschawski "Reconstitution and alignment by a magnetic field of a β-barrel membrane protein in bicelles" Eur. Biophys. J. 35:268-275 (2006) https://doi.org/10.1007/s00249-005-0014-x

17) D. E. Warschawski and P. F. Devaux "1H-13C polarization transfer in membranes: A tool for probing lipid dynamics and the effect of cholesterol" J. Magn. Reson. 177:166-171 (2005) https://doi.org/10.1016/j.jmr.2005.07.011

16) D. E. Warschawski and P. F. Devaux "Order parameters of unsaturated phospholipids in membranes and the effect of cholesterol: a 1H-13C solid-state NMR study at natural abundance" Eur. Biophys. J. 34:987-996 (2005) https://doi.org/10.1007/s00249-005-0482-z

M. N. Triba, D. E. Warschawski and P. F. Devaux "Bicelles: disks or perforated lamellae?" Eur. Biophys. J. 34:710 (2005)

15) O. Wattraint, D. E. Warschawski and C. Sarazin "Tethered or adsorbed supported lipid bilayers in nanotubes characterized by deuterium magic angle spinning NMR spectroscopy" Langmuir 21:3226-3228 (2005) https://doi.org/10.1021/la0469147

14) M.N. Triba, D. E. Warschawski and P. F. Devaux "Reinvestigation by phosphorus NMR of lipid distribution in bicelles" Biophys. J. 88:1887–1901 (2005) https://doi.org/10.1529/biophysj.104.055061

13) M. N. Triba, M. Traïkia, D. E. Warschawski, L. Nicolas-Morgantini, A. Lety, P. Gilard and P. F. Devaux "Proton Magic Angle Spinning-NMR Investigation of Surfactant Aqueous Suspensions" Journal of Colloid and Interface Science 274:341-345 (2004) https://doi.org/10.1016/j.jcis.2004.01.013

12) J.-L. Popot, E. A. Berry, D. Charvolin, C. Creuzenet, C. Ebel, D. M. Engelman, M. Flötenmeyer, F. Giusti, Y. Gohon, P. Hervé, Q. Hong, J. H. Lakey, K. Leonard, H. A. Shuman, P. Timmins, D. E. Warschawski, F. Zito, M. Zoonens, B. Pucci and C.Tribet "Amphipols: polymeric surfactants for membrane biology research" Cell. Mol. Life Sci. 60:1559-1574 (2003) https://doi.org/10.1007/s00018-003-3169-6

D. E. Warschawski and P. F. Devaux "Using DROSS to study the order of unsaturated lipid, sphingolipids and lipid / cholesterol interactions" Eur. Biophs. J. 32:288 (2003)

11) F. Ferrage, M. Zoonens, D. E. Warschawski, J.-L. Popot and G. Bodenhausen "Slow Diffusion of Macromolecular Assemblies Measured by a New Pulsed Field Gradient NMR Method" J. Am. Chem. Soc. 125:2541-2545 (2003) https://doi.org/10.1021/ja0211407

Correction: J. Am. Chem. Soc. 126:5654 (2004)

10) M. Traïkia, D. E. Warschawski, O. Lambert, J.-L. Rigaud and P. F. Devaux "Asymmetrical Membranes and Surface Tension" Biophys. J. 83:1443-1454 (2002) https://doi.org/10.1016/s0006-3495(02)73915-5

9) N. Tsapis, R. Ober, A. Chaffotte, D. E. Warschawski, J. Everett, J. Kauffman, P. Kahn, M. Waks and W. Urbach "Modification of the elastic constants of a peptide-decorated lamellar phase", Langmuir 18:4384-4392 (2002) https://doi.org/10.1021/la015745c

8) D. E. Warschawski and P.F. Devaux "Polarisation transfer in lipid membranes" J. Magn. Resonance 145:367-372 (2000) https://doi.org/10.1006/jmre.2000.2135

7) M. Traikia, D. E. Warschawski, M. Recouvreur, J. Cartaud and P. F. Devaux "Formation of unilameller vesicles by repetitive freeze-thaw cycles: characterization by electron microscopy and 31P-NMR" Eur. Biophs. J. 29:184-195 (2000) https://doi.org/10.1007/s002490000077

6) D. E. Warschawski, M. Traikia, P. F. Devaux and G. Bodenhausen, "Solid-State NMR for the study of membrane systems: The use of anisotropic interactions" Biochimie 80:437-450 (1998) https://doi.org/10.1016/s0300-9084(00)80011-5

5) D. E. Warschawski, J. D. Gross and R. G. Griffin, "Effects of membrane peptide dynamics on high-resolution magic-angle spinning NMR" Journal de Chimie Physique 95:460-466 (1998) https://doi.org/10.1051/jcp:1998160

D. E. Warschawski, J. D. Gross and R. G. Griffin, "Dynamics of gramicidin A deduced from high-resolution magic-angle spinning NMR" Biophys. J. 72:A325 (1997)

4) J. D. Gross, D. E. Warschawski and R. G. Griffin, "Dipolar recoupling in MAS NMR: A probe for segmental order in lipid bilayers" J. Am. Chem. Soc. 119:796-802 (1997) https://doi.org/10.1021/ja962951b

3) D. E. Warschawski, P. Fellmann and P. F. Devaux, "High resolution 31P-1H two-dimensional Nuclear Magnetic Resonance spectra of unsonicated lipid mixtures spinning at the magic-angle" Eur. Biophys. J. 25:131-137 (1996) https://doi.org/10.1007/s002490050024

2) J. D. Gross, P. R. Costa, J.-P. Dubacq, D. E. Warschawski, P.-N. Lirsac, P. F. Devaux and R. G. Griffin, "Multidimensional NMR in lipid systems. Coherence transfer through J couplings under MAS" J. Magn. Reson. B106:187-190 (1995) https://doi.org/10.1006/jmrb.1995.1031

1) D. E. Warschawski and P. F. Devaux, "Water suppression in 1H MASS NMR spectra of lipids and biological membranes" J. Magn. Reson. B106:76-79 (1995) https://doi.org/10.1006/jmrb.1995.1012

CONFERENCES

Organisation of the Workshop on NMR of Biological Solids in Paris (France, 2009, 2011, 2013)

REVIEWS

For: BBA - Biomembranes, Biochemistry, Biophysical Journal, Colloid and Surfaces, CRAS Chimie, European Biophysics Journal, FEBS letters, Journal of Membrane Biology, Journal of Physical Chemistry, Langmuir, Magnetic Resonance in Chemistry, Magnetic Resonance in Medicine, Phytochemical Analysis, Scientific Reports...

SKILLS

French, English, Spanish: Fluent

Computer languages used: BASIC, PASCAL, UNIX and VMS

Synthesis and purification of membrane peptides

INTERESTS

Creation of a music-band and public performances between 1984 and 1995

Traveling all over the world

Webmaster of several websites and mailing lists