Dror Emmanuel WARSCHAWSKI
Born May 25th, 1969
RESEARCH EXPERIENCE
Sorbonne Univsersité, Paris
Since 2020 Research associate at the Laboratoire des Biomolécules, CNRS - UMR
7203. Advisor: Olivier Lequin
Université du
Québec à Montréal, Québec (Canada)
2015-2019 Associate professor at the Laboratoire de RMN des systèmes biologiques
complexes. "Solid-state NMR of intact and living cells", Sabbatical,
4 years. Advisor: Isabelle Marcotte
2007 Invited professor at the Laboratoire de RMN des systèmes biologiques complexes.
"Biophysical study of bicelles: NMR, atomic force microscopy, mass
spectrometry, infra-red spectroscopy", Sabbatical, 1 year. Advisor: Isabelle
Marcotte
Institut de
Biologie Physico-Chimique, Paris
1998-2015 Research associate at the Laboratoire de Biologie Physico-Chimique des Protéines
Membranaires, CNRS - UMR 7099. Advisor: Bruno Miroux
Guelph University,
Ontario (Canada)
1997 "Structural study of membrane peptides by solid-state NMR", Post-Doctorate,
3 months. Advisor: James H. Davis
Massachusetts
Institute of Technology, Cambridge (USA)
95-97 "Lipids and membrane
peptides under physiological conditions: structural and dynamical
studies using solid-state NMR", Post-doctorate: 2 years. Advisor:
Professor Robert G. Griffin
Université
Paris Diderot, Paris (France)
92-95 "Biomembranes, model membranes: new approaches using magic-angle
spinning solid-state NMR", Ph. D. in Molecular Biophysics , 3
years. Advisor: Professor Philippe F. Devaux
Guelph University,
Ontario (Canada)
1992 "Lipid studies by solid-state NMR", 3 months. Advisor:
Professor James H. Davis
Institut de Biologie
Physico-Chimique, Paris (France)
1991 "Red blood cell study by EPR", 3 months. Advisor: Doctor
Alain Zachowski
Institut Curie,
Paris (France)
1990 "Study of an ion-plasma collision", 2 months
TEACHING EXPERIENCE
Internet
I manage the mailing list of NMR
laboratories around the world:
http://www.drorlist.com/nmr.html
http://www.drorlist.com/rmnlist.html
http://www.drorlist.com/nmrlist.html
2009-2015 and since 2020 Teaching biological NMR for the MSc "Protein
engineering and industrial applications"
Canada
2016-2019 Occasional teaching solid-state
NMR at Université
de Montréal, UQAM
(Montréal) and Université
Laval (Québec)
2006-2014 Teaching solid-state NMR for the MSc "Molecular and Cellular
Biophysics"
2005-2014 Teaching biological NMR for the MSc "Structure, Proteomics and
Functional Genetics"
2001-2014 Teaching practical NMR for the MSc in Biology
Université
Paris-Sud
11
2006 Teaching biological solid-state NMR for the MSc "Structural and
Functional Engineering of Biomolecules"
Université
Paris Descartes
2004-2006 Member of the commission de spécialistes 31-32ème section
2001-2014 Teaching solid-state NMR for the MSc "Chemical and Biological
Analyses"
Université
Paris XIII Nord
2002-2009 Member of the commission de spécialistes 28ème section
Ecole Normale
Supérieure, Paris
2006-2009 Teaching biological solid-state NMR for the MSc in Biology
2001 Teaching solid-state NMR for professors of "classes préparatoires
aux grandes écoles"
Institut de
Biologie Physico-Chimique, Paris
since 1998 Tutoring post-docs, PhD, MSc and BSc students
EDUCATION AND HONORS
Université
Paris Diderot
2006 Habilitation to Direct Researches, Physics
92-95 Recipient of a fellowship from the Ministère de l'Enseignement
Supérieur et de la Recherche for a Ph. D. in Molecular Biophysics, Cum
Magna Laude. Advisor: Philippe F. Devaux
91-92 DEA de Biophysique Moléculaire and Magistère de Physique, Honors
AB
90-91 Maitrise de Physique (Masters in Physics), Honors AB
BIBLIOGRAPHY
Research Gate
Orcid : 0000-0002-4363-4965
Scopus : 6602213358
IDREF : 154796190
HAL : 887797
47) K. Kumar, M. Sebastiao, A. A. Arnold, S. Bourgault,
D. E. Warschawski and I. Marcotte "In situ solid-state NMR study of
antimicrobial peptide interactions with erythrocyte membranes" Biophys.
J. 121:1512-1524 (2022)
https://doi.org/10.1016/j.bpj.2022.03.009
46) F. Laydevant, M. Mahabadi, P. Llido, J.-P.
Bourgouin, L. Caron, A. A. Arnold, I. Marcotte and D. E. Warschawski
"Growth-phase dependence of bacterial membrane lipid profile and
labeling for in-cell solid-state NMR applications" Biochim. Biophys.
Acta 1864:183819 (2022)
https://doi.org/10.1016/j.bbamem.2021.183819
45) A. Poulhazan, M. C. Dickwella Widanage, A. Muszyński, A. A. Arnold,
D. E. Warschawski, P. Azadi, I. Marcotte and T. Wang "Identification and
Quantification of Glycans in Whole Cells: Architecture of Microalgal
Polysaccharides Described by Solid-State Nuclear Magnetic Resonance" J.
Am. Chem. Soc. 143:19374-19388 (2021)
https://doi.org/10.1021/jacs.1c07429
44) X. Yan, K. Kumar, R. Miclette Lamarche, H. Youssef,
G. S. Shaw, I. Marcotte, C. E. DeWolf, D. E. Warschawski and E.
Boisselier "Interactions between the Cell Membrane Repair Protein
S100A10 and Phospholipid Monolayers and Bilayers" Langmuir 37:9652–9663
(2021) https://doi.org/10.1021/acs.langmuir.1c00342
43) Z. Bouhlel, A. A. Arnold, J.-S. Deschênes, J.-L.
Mouget, D. E. Warschawski, R. Tremblay and I. Marcotte "Investigating
the action of the microalgal pigment marennine on Vibrio splendidus
by in vivo 2H and 31P solid-state NMR" Biochim. Biophys.
Acta Biomembr. 1863:183642 (2021)
https://doi.org/10.1016/j.bbamem.2021.183642
42) D. E.Warschawski, K. V. Lakshmi and I. Marcotte «
Foreword to: Biophysical studies of membrane systems and interactions -
Commemorative issue in honour of Professor Michèle Auger » Biochim.
Biophys. Acta Biomembr. 1863:183609 (2021)
https://doi.org/10.1016/j.bbamem.2021.183609
41) A. Poulhazan, A. A. Arnold, D. E. Warschawski and I.
Marcotte « Solid-state NMR study of microalgal membranes and cell
walls », in Solid-state NMR: Applications in
Biomembrane Structure (F. Separovic and M. A. Sani Eds.),
Institute of Physics Publishing, Bristol, UK. Chapter 4, pp. 1-23
(2020) https://doi.org/10.1088/978-0-7503-2532-5ch4
40) A. Poulhazan, A. A. Arnold, D. E. Warschawski and I.
Marcotte "Unambiguous Ex Situ and in Cell 2D 13C Solid-State
NMR Characterization of Starch and Its Constituents" in In-Cell
NMR Spectroscopy. Biomolecular Structure and Function (A.
Shekhtman and D. S. Burz Eds.), MDPI Books, Basel, Switzerland. Chapter
8, pp. 122-138 (2020)
https://doi.org/10.3390/books978-3-03928-255-5
39) A. E. Gravel, A. A. Arnold, M. Fillion, M. Auger, D.
E. Warschawski and I. Marcotte " Magnetically-orientable Tween-based
Model Membranes for NMR Studies of Proteins" Biochim. Biophys. Acta
Biomembr. 1862:183379 (2020)
https://doi.org/10.1016/j.bbamem.2020.183379
38) X. Yan, F. Noël, I. Marcotte, C. E. DeWolf, D. E.
Warschawski and E. Boisselier "AHNAK C-Terminal Peptide Membrane
Binding-Interactions Between the Residues 5654-5673 of AHNAK and
Phospholipid Monolayers and Bilayers" Langmuir 36:362-369
(2020) https://doi.org/10.1021/acs.langmuir.9b02973
Z. Bouhlel, D. E. Warschawski, A. A. Arnold, K.
Lemarchand, R. Tremblay, and I. Marcotte "Solid-State NMR of Intact
Bacteria Reveals the Effect of Stress and Antimicrobial Agents" Biophys.
J. 118:344a (2020)
37) Z. Bouhlel, A. A. Arnold, D. E. Warschawski, K.
Lemarchand, R. Tremblay and I. Marcotte "Labelling strategy and membrane
characterization of marine bacteria Vibrio splendidus by in vivo 2H NMR"
Biochim. Biophys. Acta 1861:871-878 (2019)
https://doi.org/10.1016/j.bbamem.2019.01.018
36) A. Poulhazan, A. A. Arnold, D. E. Warschawski and I.
Marcotte "Unambiguous Ex Situ and in Cell 2D 13C Solid-State
NMR Characterization of Starch and Its Constituents" Int. J. Mol.
Sci. 19:3817 (2018)
https://doi.org/10.3390/ijms19123817
35)
E. Azar, D. Constantin and D. E. Warschawski "The effect of gramicidin
inclusions on the local order of membrane components" Eur. Phys. J.
E 41:44 (2018) https://doi.org/10.1140/epje/i2018-11644-5
34)
D. E. Warschawski, A. A. Arnold and I. Marcotte "A New Method of
Assessing Lipid Mixtures by 31P Magic-Angle Spinning NMR" Biophys.
J. 114:1368–1376 (2018)
https://doi.org/10.1016/j.bpj.2018.01.025
33) A. A.
Arnold, J.-P. Bourgouin, B. Genard, D. E. Warschawski, R. Tremblay and I
.Marcotte "Whole cell solid-state NMR study of Chlamydomonas reinhardtii
microalgae" J. Biomol. NMR 70:123-131 (2018)
https://doi.org/10.1007/s10858-018-0164-7
J.-P. Bourgouin, A.
Poulhazan, F. Zito, A. A. Arnold, D. E. Warschawski, I. Marcotte
« Methodological Development to Study Lipid Membranes of Intact
Bacteria and Microalgae by 2H Solid-State NMR » Biophys. J.
114:159a (2018)
A. A. Arnold, J.-P.
Bourgouin, B. Génard, D. E. Warschawski, F. Zito, R. Tremblay, I.
Marcotte « Whole Cell 13C Solid-State NMR of a Fully Labelled
Micro-organism: How Far Can We Go? » Biophys. J. 114:158a (2018)
A. E. Gravel, A. A. Arnold,
D. E. Warschawski, I. Marcotte « Making Soft
Magnetically-Orientable Membranes: An Alternative to Bicelles »
Biophys. J. 114:95a (2018)
32)
V. Booth, D. E. Warschawski, N. P. Santisteban, M. Laadhari and I.
Marcotte "Recent progress on the application of 2H solid-state NMR to
probe the interaction of antimicrobial peptides with intact
bacteria". Biochim. Biophys. Acta
1865:1500-1511 (2017)
https://doi.org/10.1016/j.bbapap.2017.07.018
31)
M. Beaugrand, A. A. Arnold, A. Juneau, A. Balieiro Gambaro, D. E.
Warschawski, P. T. F. Williamson and I. Marcotte "Magnetically-oriented
bicelles with monoalkylphosphocholines – versatile membrane mimetics for
Nuclear Magnetic Resonance applications". Langmuir
32:13244-13251 (2016)
https://doi.org/10.1021/acs.langmuir.6b03099
30)
X. L. Warnet, M. Laadhari, A. A. Arnold, I. Marcotte and D. E. Warschawski
"A 2H magic-angle spinning solid-state NMR characterisation of lipid
membranes in intact bacteria" Biochim. Biophys. Acta. 1858:146-152
(2016) https://doi.org/10.1016/j.bbamem.2015.10.020
29)
X. L. Warnet, A. A. Arnold, I. Marcotte and D. E. Warschawski "In-Cell
Solid-State NMR: An Emerging Technique for the Study of Biological
Membranes" Biophys.
J. 109:2461–2466 (2015)
https://doi.org/10.1016/j.bpj.2015.10.041
28)
G. Hattab, D. E. Warschawski, K. Moncoq and B. Miroux "Escherichia coli as
host for membrane protein structure determination: a global analysis" Sci. Rep. 5:12097 (2015)
https://doi.org/10.1038/srep12097
27)
A. A. Arnold, B. Genard, F. Zito, R. Tremblay, D. E. Warschawski and I.
Marcotte "Identification of lipid and saccharide constituents of whole
microalgal cells by 13C solid-state NMR" Biochim.
Biophys. Acta 1848:369-377
(2015) https://doi.org/10.1016/j.bbamem.2014.07.017
26)
G. Hattab, A. Y. T. Suisse, O. Ilioaia, M. Casiraghi, M. Dezi, X. L.
Warnet, D. E. Warschawski, K. Moncoq, M. Zoonens and B. Miroux "Membrane
Protein Production in Escherichia coli: Overview and Protocols"
In Membrane Proteins Production
for Structural Analysis. I. Mus-Veteau (Ed.), Springer,
New-York, USA. Chapter 4, pp.
87-106 (2014) https://doi.org/10.1007/978-1-4939-0662-8_4
25)
L. J. Catoire, X. L. Warnet and D. E. Warschawski "Micelles, Bicelles,
Amphipols, Nanodiscs, Liposomes, or Intact Cells: The Hitchhiker’s Guide
to the Study of Membrane Proteins by NMR" In
Membrane Proteins Production for Structural Analysis. I.
Mus-Veteau (Ed.), Springer, New-York, USA. Chapter
12, pp. 315-345 (2014) https://doi.org/10.1007/978-1-4939-0662-8_12
24)
M. Beaugrand, A. A. Arnold, J. Hénin, D. E. Warschawski, P. T. F.
Williamson, and I. Marcotte "Lipid Concentration and Molar Ratio
Boundaries for the Use of Isotropic Bicelles"
Langmuir 30:6162-6170
(2014) https://doi.org/10.1021/la5004353
23) A. Abdine,
K.-H. Park and D. E. Warschawski "Cell-free membrane protein expression
for solid-state NMR" Methods Mol. Biol. 831:85-109
(2012) https://doi.org/10.1007/978-1-61779-480-3_6
22)
D. E. Warschawski, A. A. Arnold, M. Beaugrand, A. Gravel, E. Chartrand and
I. Marcotte "Choosing membrane mimetics for NMR structural studies of
transmembrane proteins" Biochim.
Biophys.
Acta 1808:1957-1974
(2011) https://doi.org/10.1016/j.bbamem.2011.03.016
21) A. Abdine, M. A. Verhoeven and D. E. Warschawski "Cell-free
expression and labeling strategies for a new decade in solid-state NMR"
New Biotechnol. 28:272-276
(2011) https://doi.org/10.1016/j.nbt.2010.07.014
20) A.
Abdine, M. A. Verhoeven, K.-H. Park, A. Ghazi, E. Guittet, C. Berrier,
C. Van Heijenoort and D. E. Warschawski "Structural study of the
membrane protein MscL using cell-free expression and solid-state NMR" J. Magn. Reson. 204:155-159
(2010) https://doi.org/10.1016/j.jmr.2010.02.003
19) M. N.
Triba, P. F. Devaux and D. E. Warschawski "Effects of lipid chain length
and unsaturation on bicelles stability. A phosphorus NMR study" Biophys.
J. 91:1357-1367 (2006)
https://doi.org/10.1529/biophysj.106.085118
18) M. N.
Triba, M. Zoonens, J.-L. Popot, P. F. Devaux and D. E. Warschawski
"Reconstitution and alignment by a magnetic field of a β-barrel membrane
protein in bicelles" Eur. Biophys. J. 35:268-275
(2006) https://doi.org/10.1007/s00249-005-0014-x
17)
D. E. Warschawski and P. F. Devaux "1H-13C polarization transfer in
membranes: A tool for probing lipid dynamics and the effect of
cholesterol" J. Magn. Reson. 177:166-171 (2005)
https://doi.org/10.1016/j.jmr.2005.07.011
16)
D. E. Warschawski and P. F. Devaux "Order parameters of unsaturated
phospholipids in membranes and the effect of cholesterol: a 1H-13C
solid-state NMR study at natural abundance" Eur. Biophys. J. 34:987-996
(2005) https://doi.org/10.1007/s00249-005-0482-z
M.
N. Triba, D. E. Warschawski and P. F. Devaux "Bicelles: disks or
perforated lamellae?" Eur. Biophys. J. 34:710 (2005)
15) O.
Wattraint, D. E. Warschawski and C. Sarazin "Tethered or adsorbed
supported lipid bilayers in nanotubes characterized by deuterium magic
angle spinning NMR spectroscopy" Langmuir 21:3226-3228
(2005)
https://doi.org/10.1021/la0469147
14) M.N.
Triba, D. E. Warschawski and P. F. Devaux "Reinvestigation by phosphorus
NMR of lipid distribution in bicelles" Biophys. J. 88:1887–1901
(2005) https://doi.org/10.1529/biophysj.104.055061
13) M. N.
Triba, M. Traïkia, D. E. Warschawski, L. Nicolas-Morgantini, A. Lety, P.
Gilard and P. F. Devaux "Proton Magic Angle Spinning-NMR Investigation
of Surfactant Aqueous Suspensions" Journal of Colloid and Interface
Science 274:341-345 (2004)
https://doi.org/10.1016/j.jcis.2004.01.013
12) J.-L. Popot,
E. A. Berry, D. Charvolin, C. Creuzenet, C. Ebel, D. M. Engelman, M.
Flötenmeyer, F. Giusti, Y. Gohon, P. Hervé, Q. Hong, J. H. Lakey, K.
Leonard, H. A. Shuman, P. Timmins, D. E. Warschawski, F. Zito, M. Zoonens,
B. Pucci and C.Tribet "Amphipols: polymeric surfactants for membrane
biology research" Cell. Mol. Life Sci. 60:1559-1574 (2003)
https://doi.org/10.1007/s00018-003-3169-6
D.
E. Warschawski and P. F. Devaux "Using DROSS to study the order of
unsaturated lipid, sphingolipids and lipid / cholesterol interactions" Eur.
Biophs.
J. 32:288 (2003)
11) F.
Ferrage, M. Zoonens, D. E. Warschawski, J.-L. Popot and G. Bodenhausen
"Slow Diffusion of Macromolecular Assemblies Measured by a New Pulsed
Field Gradient NMR Method" J. Am. Chem. Soc. 125:2541-2545
(2003) https://doi.org/10.1021/ja0211407
Correction: J.
Am. Chem. Soc.
126:5654 (2004)
10) M.
Traïkia, D. E. Warschawski, O. Lambert, J.-L. Rigaud and P. F. Devaux
"Asymmetrical Membranes and Surface Tension" Biophys. J. 83:1443-1454
(2002) https://doi.org/10.1016/s0006-3495(02)73915-5
9) N.
Tsapis, R. Ober, A. Chaffotte, D. E. Warschawski, J. Everett, J.
Kauffman, P. Kahn, M. Waks and W. Urbach "Modification of the elastic
constants of a peptide-decorated lamellar phase", Langmuir 18:4384-4392
(2002) https://doi.org/10.1021/la015745c
8) D.
E. Warschawski and P.F. Devaux "Polarisation transfer in lipid
membranes" J. Magn. Resonance 145:367-372 (2000)
https://doi.org/10.1006/jmre.2000.2135
7) M.
Traikia, D. E. Warschawski, M. Recouvreur, J. Cartaud and P. F. Devaux
"Formation of unilameller vesicles by repetitive freeze-thaw cycles:
characterization by electron microscopy and 31P-NMR" Eur. Biophs. J.
29:184-195 (2000) https://doi.org/10.1007/s002490000077
6) D.
E. Warschawski, M. Traikia, P. F. Devaux and G. Bodenhausen,
"Solid-State NMR for the study of membrane systems: The use of
anisotropic interactions" Biochimie 80:437-450
(1998) https://doi.org/10.1016/s0300-9084(00)80011-5
5) D.
E. Warschawski, J. D. Gross and R. G. Griffin, "Effects of membrane
peptide dynamics on high-resolution magic-angle spinning NMR" Journal
de Chimie Physique 95:460-466 (1998)
https://doi.org/10.1051/jcp:1998160
D.
E. Warschawski, J. D. Gross and R. G. Griffin, "Dynamics of gramicidin A
deduced from high-resolution magic-angle spinning NMR" Biophys. J.
72:A325 (1997)
4) J. D.
Gross, D. E. Warschawski and R. G. Griffin, "Dipolar recoupling in MAS
NMR: A probe for segmental order in lipid bilayers" J. Am. Chem.
Soc. 119:796-802 (1997) https://doi.org/10.1021/ja962951b
3) D.
E. Warschawski, P. Fellmann and P. F. Devaux, "High resolution 31P-1H
two-dimensional Nuclear Magnetic Resonance spectra of unsonicated lipid
mixtures spinning at the magic-angle" Eur. Biophys. J. 25:131-137
(1996) https://doi.org/10.1007/s002490050024
2) J. D.
Gross, P. R. Costa, J.-P. Dubacq, D. E. Warschawski, P.-N. Lirsac, P. F.
Devaux and R. G. Griffin, "Multidimensional NMR in lipid systems.
Coherence transfer through J couplings under MAS" J. Magn.
Reson. B106:187-190 (1995)
https://doi.org/10.1006/jmrb.1995.1031
1) D.
E. Warschawski and P. F. Devaux, "Water suppression in 1H MASS NMR
spectra of lipids and biological membranes" J. Magn. Reson. B106:76-79
(1995) https://doi.org/10.1006/jmrb.1995.1012
CONFERENCES
Organisation of the Workshop
on NMR of Biological Solids in Paris (France, 2009, 2011,
2013)
REVIEWS
For: BBA - Biomembranes, Biochemistry, Biophysical Journal, Colloid and
Surfaces, CRAS Chimie, European Biophysics Journal, FEBS letters, Journal
of Membrane Biology, Journal of Physical
Chemistry, Langmuir, Magnetic Resonance in Chemistry, Magnetic Resonance
in Medicine, Phytochemical Analysis, Scientific Reports...
SKILLS
French, English, Spanish: Fluent
Computer languages used: BASIC, PASCAL, UNIX and VMS
Synthesis and purification of membrane peptides
INTERESTS
Creation of a music-band and public performances between
1984 and 1995
Traveling all over the world
Webmaster of several websites and mailing lists