Dror Emmanuel WARSCHAWSKI
Born May 25th, 1969
RESEARCH EXPERIENCE
Université
du Québec à Montréal, Québec (Canada)
Since 2015 Associate professor. "Solid-state NMR of intact and living
cells", Sabbatical, 3 years. Advisor: Isabelle Marcotte
2007 "Biophysical study of bicelles: NMR, atomic force microscopy, mass
spectrometry, infra-red spectroscopy", Sabbatical, 1 year. Advisor:
Isabelle Marcotte
Institut de
Biologie Physico-Chimique, Paris (France)
Since 1998 Research associate at the Laboratoire de Biologie
Physico-Chimique des Protéines Membranaires, CNRS - UMR 7099. Advisor: Bruno Miroux.
Guelph University,
Ontario (Canada)
1997 "Structural study of membrane peptides by solid-state NMR", Post-Doctorate,
3 months. Advisor: James H. Davis
Massachusetts
Institute of Technology, Cambridge (USA)
95-97 "Lipids and membrane
peptides under physiological conditions: structural and dynamical
studies using solid-state NMR", Post-doctorate: 2 years. Advisor:
Professor Robert G. Griffin
Université
Paris Diderot, Paris (France)
92-95 "Biomembranes, model membranes: new approaches using magic-angle
spinning solid-state NMR", Ph. D. in Molecular Biophysics , 3
years. Advisor: Professor Philippe F. Devaux
Guelph University,
Ontario (Canada)
1992 "Lipid studies by solid-state NMR", 3 months. Advisor:
Professor James H. Davis
Institut de Biologie
Physico-Chimique, Paris (France)
1991 "Red blood cell study by EPR", 3 months. Advisor: Doctor
Alain Zachowski
Institut Curie,
Paris (France)
1990 "Study of an ion-plasma collision", 2 months
TEACHING EXPERIENCE
On the internet
I manage the mailing list of NMR laboratories around the
world, as well as the two databases for protein structures determined by
NMR:
Canada
2016 Occasional teaching solid-state NMR at UQAM
(Montréal) and Université
Laval (Québec)
2009-2015 Teaching biological NMR for the MSc "Protein engineering and
industrial applications"
2006-2014 Teaching solid-state NMR for the MSc "Molecular and Cellular
Biophysics"
2005-2014 Teaching biological NMR for the MSc "Structure, Proteomics and
Functional Genetics"
2001-2014 Teaching practical NMR for the MSc in Biology
Université
Paris-Sud
11
2006 Teaching biological solid-state NMR for the MSc "Structural and
Functional Engineering of Biomolecules"
Université
Paris Descartes
2004-2006 Member of the commission de spécialistes 31-32ème section
2001-2014 Teaching solid-state NMR for the MSc "Chemical and Biological
Analyses"
Université
Paris XIII Nord
2002-2009 Member of the commission de spécialistes 28ème section
Ecole Normale
Supérieure, Paris
2006-2009 Teaching biological solid-state NMR for the MSc in Biology
2001 Teaching solid-state NMR for professors of "classes préparatoires
aux grandes écoles"
Institut de
Biologie Physico-Chimique, Paris
since 1998 Tutoring post-docs, PhD, MSc and BSc students
EDUCATION AND HONORS
Université
Paris Diderot
2006 Habilitation to Direct Researches, Physics
92-95 Recipient of a fellowship from the Ministère de l'Enseignement
Supérieur et de la Recherche for a Ph. D. in Molecular Biophysics, Cum
Magna Laude. Advisor: Philippe F. Devaux
91-92 DEA de Biophysique Moléculaire and Magistère de Physique, Honors
AB
90-91 Maitrise de Physique (Masters in Physics), Honors AB
BIBLIOGRAPHY
36)
E. Azar, D. Constantin and D. E. Warschawski "The effect of gramicidin
inclusions on the local order of membrane components" Eur. Phys. J.
E 41:44 (2018)
35)
D. E. Warschawski, A. A. Arnold and I. Marcotte "A New Method of
Assessing Lipid Mixtures by 31P Magic-Angle Spinning NMR" Biophys.
J. 114:1368–1376 (2018)
34) A. A.
Arnold, J.-P. Bourgouin, B. Genard, D. E. Warschawski, R. Tremblay and I
.Marcotte "Whole cell solid-state NMR study of Chlamydomonas reinhardtii
microalgae" J. Biomol. NMR 70:123-131 (2018)
33)
V. Booth, D. E. Warschawski, N. P. Santisteban, M. Laadhari and I.
Marcotte"Recent progress on the application of 2H solid-state NMR to
probe the interaction of antimicrobial peptides with intact
bacteria". Biochim. Biophys. Acta
1865:1500-1511 (2017)
32)
M. Beaugrand, A. A. Arnold, A. Juneau, A. Balieiro Gambaro, D. E.
Warschawski, P. T. F. Williamson and I. Marcotte "Magnetically-oriented
bicelles with monoalkylphosphocholines – versatile membrane mimetics for
Nuclear Magnetic Resonance applications". Langmuir
32:13244-13251 (2016)
X. L. Warnet, A. A. Arnold, I. Marcotte and D. E.
Warschawski "NMR can now scrutinize the membrane of a living cell"
Atlas of Science, 16
June (2016)
31)
X. L. Warnet, M. Laadhari, A. A. Arnold, I. Marcotte and D. E. Warschawski
"A 2H magic-angle spinning solid-state NMR characterisation of lipid
membranes in intact bacteria" Biochim. Biophys. Acta. 1858:146-152
(2016)
30)
X. L. Warnet, A. A. Arnold, I. Marcotte and D. E. Warschawski "In-Cell
Solid-State NMR: An Emerging Technique for the Study of Biological
Membranes" Biophys.
J. 109:2461–2466 (2015)
29)
G. Hattab, D. E. Warschawski, K. Moncoq and B. Miroux "Escherichia coli as
host for membrane protein structure determination: a global analysis" Sci. Rep. 5:12097 (2015)
28)
A. A. Arnold, B. Genard, F. Zito, R. Tremblay, D. E. Warschawski and I.
Marcotte "Identification of lipid and saccharide constituents of whole
microalgal cells by 13C solid-state NMR" Biochim.
Biophys. Acta 1848:369-377
(2015)
27)
G. Hattab, A. Y. T. Suisse, O. Ilioaia, M. Casiraghi, M. Dezi, X. L.
Warnet, D. E. Warschawski, K. Moncoq, M. Zoonens and B. Miroux "Membrane
Protein Production in Escherichia coli: Overview and Protocols"
In Membrane Proteins Production
for Structural Analysis. I. Mus-Veteau (Ed.), Springer,
New-York, USA. Chapter 4, pp.
87-106 (2014)
26)
L. J. Catoire, X. L. Warnet and D. E. Warschawski "Micelles, Bicelles,
Amphipols, Nanodiscs, Liposomes, or Intact Cells: The Hitchhiker’s Guide
to the Study of Membrane Proteins by NMR" In
Membrane Proteins Production for Structural Analysis. I.
Mus-Veteau (Ed.), Springer, New-York, USA. Chapter
12, pp. 315-345 (2014)
25) M. Beaugrand, A. A. Arnold, J. Hénin, D. E. Warschawski, P. T. F.
Williamson, and I. Marcotte "Lipid Concentration and Molar Ratio
Boundaries for the Use of Isotropic Bicelles"
Langmuir 30:6162-6170
(2014)
D.
Warschawski "La fin du monde: la faute à qui?" Le
Courrier (Genève), mardi 6 mai,
page 4 (2014)
24)
A. A. Arnold, B. Genard, A. L. D. A. G. Da Silva, R. Tremblay, D. E.
Warschawski, F. Zito and I. Marcotte "Screening the Dynamics of Membrane
Constituents in Intact Microalgal Cells by Solid-State NMR" Biophys.
J. 106:508a (2014)
G.
Hattab,
K. Moncoq, D. Warschawski and B. Miroux "Escherichia Coli as Host for
Membrane Protein Structure Determination: A Global Analysis" Biophys.
J. 106:46a (2014)
23) A. Abdine,
K.-H. Park and D. E. Warschawski "Cell-free membrane protein expression
for solid-state NMR" Methods Mol. Biol. 831:85-109
(2012)
22)
D. E. Warschawski, A. A. Arnold, M. Beaugrand, A. Gravel, E. Chartrand and
I. Marcotte "Choosing membrane mimetics for NMR structural studies of
transmembrane proteins" Biochim.
Biophys.
Acta 1808:1957-1974
(2011)
21) A. Abdine, M. A. Verhoeven and D. E. Warschawski "Cell-free
expression and labeling strategies for a new decade in solid-state NMR"
New Biotechnol. 28:272-276
(2011)
20) A.
Abdine, M. A. Verhoeven, K.-H. Park, A. Ghazi, E. Guittet, C. Berrier,
C. Van Heijenoort and D. E. Warschawski "Structural study of the
membrane protein MscL using cell-free expression and solid-state NMR" J. Magn. Reson. 204:155-159
(2010)
19) M. N.
Triba, P. F. Devaux and D. E. Warschawski "Effects of lipid chain length
and unsaturation on bicelles stability. A phosphorus NMR study" Biophys.
J. 91:1357-1367 (2006)
18) M. N.
Triba, M. Zoonens, J.-L. Popot, P. F. Devaux and D. E. Warschawski
"Reconstitution and alignment by a magnetic field of a β-barrel membrane
protein in bicelles" Eur. Biophys. J. 35:268-275
(2006)
17)
D. E. Warschawski and P. F. Devaux "1H-13C polarization transfer in
membranes: A tool for probing lipid dynamics and the effect of
cholesterol" J. Magn. Reson. 177:166-171 (2005)
16)
D. E. Warschawski and P. F. Devaux "Order parameters of unsaturated
phospholipids in membranes and the effect of cholesterol: a 1H-13C
solid-state NMR study at natural abundance" Eur. Biophys. J. 34:987-996
(2005)
M. N.
Triba, D. E. Warschawski and P. F. Devaux "Bicelles: disks or perforated
lamellae?" Eur. Biophys. J. 34:710 (2005)
15) O.
Wattraint, D. E. Warschawski and C. Sarazin "Tethered or adsorbed
supported lipid bilayers in nanotubes characterized by deuterium magic
angle spinning NMR spectroscopy" Langmuir 21:3226-3228
(2005)
14) M.N.
Triba, D. E. Warschawski and P. F. Devaux "Reinvestigation by phosphorus
NMR of lipid distribution in bicelles" Biophys. J. 88:1887–1901
(2005)
13) M. N.
Triba, M. Traïkia, D. E. Warschawski, L. Nicolas-Morgantini, A. Lety, P.
Gilard and P. F. Devaux "Proton Magic Angle Spinning-NMR Investigation
of Surfactant Aqueous Suspensions" Journal of Colloid and Interface
Science 274:341-345 (2004)
12) J.-L. Popot,
E. A. Berry, D. Charvolin, C. Creuzenet, C. Ebel, D. M. Engelman, M.
Flötenmeyer, F. Giusti, Y. Gohon, P. Hervé, Q. Hong, J. H. Lakey, K.
Leonard, H. A. Shuman, P. Timmins, D. E. Warschawski, F. Zito, M. Zoonens,
B. Pucci and C.Tribet "Amphipols: polymeric surfactants for membrane
biology research" Cell. Mol. Life Sci. 60:1559-1574 (2003)
D. E.
Warschawski and P. F. Devaux "Using DROSS to study the order of
unsaturated lipid, sphingolipids and lipid / cholesterol interactions" Eur.
Biophs.
J. 32:288 (2003)
11) F.
Ferrage, M. Zoonens, D. E. Warschawski, J.-L. Popot and G. Bodenhausen
"Slow Diffusion of Macromolecular Assemblies Measured by a New Pulsed
Field Gradient NMR Method" J. Am. Chem. Soc. 125:2541-2545
(2003)
Correction: J.
Am. Chem. Soc.
126:5654 (2004)
10) M.
Traïkia, D. E. Warschawski, O. Lambert, J.-L. Rigaud and P. F. Devaux
"Asymmetrical Membranes and Surface Tension" Biophys. J. 83:1443-1454
(2002)
9) N.
Tsapis, R. Ober, A. Chaffotte, D. E. Warschawski, J. Everett, J.
Kauffman, P. Kahn, M. Waks and W. Urbach "Modification of the elastic
constants of a peptide-decorated lamellar phase", Langmuir 18:4384-4392
(2002)
8) D.
E. Warschawski and P.F. Devaux "Polarisation transfer in lipid
membranes" J. Magn. Resonance 145:367-372 (2000)
7) M.
Traikia, D. E. Warschawski, M. Recouvreur, J. Cartaud and P. F. Devaux
"Formation of unilameller vesicles by repetitive freeze-thaw cycles:
characterization by electron microscopy and 31P-NMR" Eur. Biophs. J.
29:184-195 (2000)
6) D.
E. Warschawski, M. Traikia, P. F. Devaux and G. Bodenhausen,
"Solid-State NMR for the study of membrane systems: The use of
anisotropic interactions" Biochimie 80:437-450
(1998)
5) D.
E. Warschawski, J. D. Gross and R. G. Griffin, "Effects of membrane
peptide dynamics on high-resolution magic-angle spinning NMR" Journal
de Chimie Physique 95:460-466 (1998)
D. E.
Warschawski, J. D. Gross and R. G. Griffin, "Dynamics of gramicidin A
deduced from high-resolution magic-angle spinning NMR" Biophys. J.
72:A325 (1997)
4) J. D.
Gross, D. E. Warschawski and R. G. Griffin, "Dipolar recoupling in MAS
NMR: A probe for segmental order in lipid bilayers" J. Am. Chem.
Soc. 119:796-802 (1997)
3) D.
E. Warschawski, P. Fellmann and P. F. Devaux, "High resolution 31P-1H
two-dimensional Nuclear Magnetic Resonance spectra of unsonicated lipid
mixtures spinning at the magic-angle" Eur. Biophys. J. 25:131-137
(1996)
2) J. D.
Gross, P. R. Costa, J.-P. Dubacq, D. E. Warschawski, P.-N. Lirsac, P. F.
Devaux and R. G. Griffin, "Multidimensional NMR in lipid systems.
Coherence transfer through J couplings under MAS" J. Magn.
Reson. B106:187-190 (1995)
1) D.
E. Warschawski and P. F. Devaux, "Water suppression in 1H MASS NMR
spectra of lipids and biological membranes" J. Magn. Reson. B106:76-79
(1995)
CONFERENCES
Organisation of the Workshop
on NMR of Biological Solids in Paris (France, 2009, 2011,
2013)
REVIEWS
For: Biochemistry, Biophysical Journal, Colloid and Surfaces, CRAS
Chimie, European Biophysics Journal, FEBS letters, Journal of
Membrane Biology, Magnetic Resonance in Chemistry, Magnetic Resonance in
Medicine...
SKILLS
French, English, Hebrew, Spanish: Fluent
Computer languages used: BASIC, PASCAL, UNIX and VMS
Synthesis and purification of membrane peptides
INTERESTS
Creation of a music-band and public performances between
1984 and 1995
Traveling all over the world
Webmaster of several websites and mailing lists